Cells were plated at 100,000 cells per well in 6 well plates, 3 wells per treatment. Cells were transfected using Lipofectamine 2000 and the manufacturers associated protocol with siRNA concentrations of 150 nM. Cells were incubated for 4 hours before media was added to dilute the transfection media. CellEvent Caspase 3/7 reagent (Invitrogen) was applied, per manufacturers protocol, post transfection and imaged using an EVOS FL 2 Auto Imaging System.
Fl 2 auto imaging system
Manufactured by Thermo Fisher Scientific
The FL 2 Auto Imaging System is a laboratory instrument designed for automated fluorescence imaging. It provides a standardized and consistent approach to capturing and analyzing fluorescent signals from various samples.
Automatically generated - may contain errors
2 protocols using fl 2 auto imaging system
1
Caspase 3/7 Apoptosis Assay
2
Caspase 3/7 Activity Assay
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