AP, 4HA, and AP-d8 were obtained as solutions from Cerilliant (Austin, TX, USA). HPLC-grade methanol was purchased from J.T. Baker/Avantor (Center Valley, PA, USA). Water (LiChrosolv-grade) was supplied by Merck (Darmstadt, Germany). Formic acid (LC–MS LiChropur-grade) was purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals were of analytical grade or higher. The AP and 4HA working standard solutions (10 µg/mL) were prepared by dilution with methanol. The IS working solution was prepared in methanol to obtain an AP-d8 solution (0.1 µg/mL). These solutions were stored at −20 °C in amber bottles before use.
Water lichrosolv grade
Manufactured by Merck Group
Sourced in Germany
LiChrosolv-grade water is a high-purity laboratory reagent water that meets the specifications for the LiChrosolv brand. It is designed for use in analytical applications, particularly in liquid chromatography (LC) and other sensitive analytical techniques where high-quality solvent is required. The water is processed to remove impurities and meets the quality standards for the LiChrosolv product line.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade methanol, by Avantor (2 mentions)
Formic acid lc ms lichropur grade, by Merck Group (2 mentions)
Ap d8, by Cerilliant (2 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
2 propanol, by Merck Group (1 mentions)
Ammonium formate, by Thermo Fisher Scientific (1 mentions)
Pooled human plasma, by Biowest (1 mentions)
Formic acid, by Merck Group (1 mentions)
3 protocols using water lichrosolv grade
1
Quantitative Analysis of AP and 4HA
2
Quantitative Analysis of AP and 4HA
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AP, 4HA, and AP-d8 were obtained as solutions from Cerilliant (Austin, TX, USA). HPLC-grade methanol was purchased from J.T. Baker/Avantor (Center Valley, PA, USA). Water (LiChrosolv-grade) was supplied by Merck (Darmstadt, Germany). Formic acid (LC–MS LiChropur-grade) was purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals were of analytical grade or higher. The AP and 4HA working standard solutions (10 µg/mL) were prepared by dilution with methanol. The IS working solution was prepared in methanol to obtain an AP-d8 solution (0.1 µg/mL). These solutions were stored at −20 °C in amber bottles before use.
3
Plasma Sample Preparation for LC-MS
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Acetonitrile (ACN), methanol, formic acid and 2-propanol (IPA), methyl-tert-butyl-ether (MTBE), all LC-MS grade, were purchased from Sigma-Aldrich (Steinheim, Germany). Water, LiChrosolv grade, was obtained from Merck (Darmstadt, Germany). Ammonium formate, 99% crystalline, was from Alfa Aesar (Karlsruhe, Germany). Pooled human plasma was purchased from Biowest (Nuaillé, France).
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