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2 protocols using costunolide

1

Synthetic Strigolactone and Costunolide Treatments

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Synthetic strigolactone GR24 (C17H14O5) was purchased from B. Zwanenburg, Department of Organic Chemistry, Radboud University Nijmegen, Nijmegen, The Netherlands. GR24 was used at a concentration of 1 ppm (= 3.3 × 10–6 M) in an aqueous solution. Costunolide (C15H20O2) was purchased from Selleck Chemicals (Houston, TX, United States), dissolved, and diluted in methanol.
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2

Costunolide and Bile Acid Analysis

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Costunolide was purchased from Selleck (Shanghai, China). Reagents and standards, including DDC (137030), cholic acid (CA, C1129), taurocholic acid (TCA, T4009), glycocholic acid (GCA, G2878), α-muricholic acid (α-MCA, 700232P), tauro α-muricholic acid (T-α-MCA, 700243P), β-muricholic acid (β-MCA, SML2372), tauro β-muricholic acid (T-β-MCA, 700244P), chenodeoxy cholic acid (CDCA, C9377), taurochenodeoxycholic acid (TCDCA, T6260), deoxycholic acid (DCA, D2510), taurodeoxycholic acid (TDCA, T0875), ursodeoxycholic acid (UDCA, U5127), tauroursodeoxycholic acid (TUDCA, T0266), lithocholic acid (LCA, L6250), taurolithocholic acid (TLCA, T7515), ω-muricholic acid (β-MCA, 700231P), and tauro ω-muricholic acid (T-ω-MCA, 700245P), were purchased from Sigma–Aldrich. All other chemicals and reagents were obtained from commercial sources.
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