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4 protocols using loratadine

1

Loratadine's Anti-Inflammatory Effects

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Loratadine was purchased from Tokyo Chemical Industry Co., LTD. (Tokyo, Japan). Carboxymethylcellulose (CMC), dimethylsulfoxide (DMSO), L-NAME, lipopolysaccharide, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT), polyethyleneimine (PEI), Ranitidine, and stillen were obtained from Sigma Aldrich (St. Louis, MO, USA). DMEM, fetal bovine serum (FBS), penicillin/streptomycin, phosphate-buffered saline (PBS), RPMI 1640, trypsin, and TRIzol were acquired from GIBCO (Grand Island, NY, USA). HEK293T (CRL-1573) and RAW264.7 cells (CRL-2278) were products from American Type Culture Collection (Rockville, MD, USA). Luciferase constructs with AP-1 binding sites and CFP-TRIF, FLAG-MYD88, pcDNA, and Tag-2 constructs were used as previously reported [86 (link)]. Real-time and semiquantitative reverse transcriptase-polymerase chain reaction primers were bought from Macrogen Inc. (Seoul, Korea). All other chemicals were purchased from Sigma. Total and phospho antibodies for the MAPK family were obtained from Cell Signaling Technology (Beverly, MA, USA) and Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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2

Diverse Pharmacological Modulators of Inflammatory Pathways

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Montelukast, pranlukast and zafirlukast as leukotriene receptor antagonists were purchased from Cayman Chemical (Ann Arbor, MI, USA). Suplatast tosilate as a Th2 cytokine inhibitor, sodium cromoglicate, tranilast, amlexanox and pemirolast as mediator release inhibitors, ozagrel as a thromboxane A2 synthesis inhibitor, seratrodast as a thromboxane A 2 receptor antagonist, diphenhydramine as an ethanolamine H1 blocker, chlorpheniramine as a propylamine H1 blocker, cyproheptadine as a piperidine H1 blocker, promethazine as a phenothiazine H1 blocker, and ketotifen, fexofenadine, epinastine, cetirizine, levocetirizine, emedastine, loratadine, azelastine, mequitazine and ebastine as second-generation H1 receptor antagonists were purchased from Tokyo Chemical Industry (Tokyo, Japan).
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3

Synthesis and Characterization of Bioactive Compounds

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Acetaminophen and 1-aminobenzotriazole (ABT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Amitriptyline hydrochloride, loratadine, and desloratadine were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Amiodarone was purchased from LKT Laboratories, Inc. (St. Paul, MN, USA). NBD-PE was purchased from Setareh Biotech, LLC (Eugene, OR, USA). LysoTracker Red ® DND-99 was purchased from Life Technologies (Eugene, OR, USA).
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4

Comprehensive Catalog of Pharmaceutical Compounds

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Amikacin, caffeine, citrate, clozapine, dexamethasone, glimepiride, imipramine, ketotifen, lactose, sorbitol, and theophylline were purchased from Wako Pure Chemical Industries (Osaka, Japan). 3-Acetamidophenol, amiodarone, amitriptyline, ascorbate, perhexiline, and tamoxifen were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chlorpromazine, desloratadine, isoproterenol, loratadine, maprotiline, and tilorone were purchased from Tokyo Chemical Industries (Tokyo, Japan). All compounds were of the highest available commercial grade.
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