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Phospho pdgfr β

Manufactured by Santa Cruz Biotechnology
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Phospho-PDGFR-β is an antibody that detects the phosphorylated form of the beta-type platelet-derived growth factor receptor (PDGFR-β). It is used for the detection and quantification of PDGFR-β phosphorylation in various applications, such as Western blotting and immunoprecipitation.

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Lab products found in correlation

C anp4 23, by Bachem (1 mentions) Total egfr, by Santa Cruz Biotechnology (1 mentions) Methyl 3h thymidine, by PerkinElmer (1 mentions) C src, by Santa Cruz Biotechnology (1 mentions) Phospho egfr, by Santa Cruz Biotechnology (1 mentions) Total pdgfr β, by Santa Cruz Biotechnology (1 mentions) Pdgfr β, by Santa Cruz Biotechnology (1 mentions) Bcl xl h 62, by Santa Cruz Biotechnology (1 mentions) Shp1 c19, by Santa Cruz Biotechnology (1 mentions) Phospho akt, by Santa Cruz Biotechnology (1 mentions) Mini protean tgx precast protein gel, by Bio-Rad (1 mentions) Phospho mek1 2, by Cell Signaling Technology (1 mentions) Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions) Pathscan rtk signaling antibody array kit, by Cell Signaling Technology (1 mentions) Lc3b map1lc3b, by Novus Biologicals (1 mentions) Trans blot turbo midi pvdf transfer pack, by Bio-Rad (1 mentions) P44 42 mapk, by Cell Signaling Technology (1 mentions) Irdye 680rd, by LI COR (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

PDGFR-β PDGFR

3 protocols using phospho pdgfr β

1

Signaling Pathway Protein Analysis

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C-ANP 4–23 was purchased from Bachem, antibodies against Cyclin D1, Cdk4, total EGF-R, phospho-EGFR, total PDGF-R β, phospho-PDGF-R β, total IGF-R β, phospho-IGF-IR, c-Src, phosphor-c-Src, horseradish peroxidase-conjugated goat anti-mouse/anti-rabbit and anti-goat immunoglobulin were from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA), antibodies against Nox1, Nox2, Nox4 and p47phox were from EMD Millipore. Thymidine, [Methyl-3H] was from PerkinElmer, Inc. (Massachusetts, U.S.A.). All other chemicals were purchased from Sigma Aldrich Canada.
Rahali S., Li Y, & Anand-Srivastava M.B. (2018). Contribution of oxidative stress and growth factor receptor transactivation in natriuretic peptide receptor C-mediated attenuation of hyperproliferation of vascular smooth muscle cells from SHR. PLoS ONE, 13(1), e0191743.
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2

Immunoblotting Primary Antibody Optimization

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Primary antibodies for immunoblotting were purchased from commercial sources: actin (horseradish peroxidase; I-19), GAPDH horseradish peroxidase (V18), SHP-1 (C19), PDGF-β (N30), and Bcl-xL (H-62) from Santa Cruz Biotechnology Inc (Dallas, TX); protein kinase B (Akt), phospho-Akt (D9E), phospho-PDGFR-β (Y1009), PDGFR-β, phospho-ERK (extracellular signal-regulated kinase), ERK and secondary antibody of anti-rabbit and anti-mouse
Mercier C., Brazeau T., Lamoureux J., Boisvert E., Robillard S., Breton V., Paré M., Guay A., Lizotte F., Despatis M.A, & Geraldes P. (2021). Diabetes Impaired Ischemia-Induced PDGF (Platelet-Derived Growth Factor) Signaling Actions and Vessel Formation Through the Activation of Scr Homology 2-Containing Phosphatase-1. Arteriosclerosis, thrombosis, and vascular biology, 41(9).
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3

Western Blotting Protocol for Diverse Protein Analysis

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For western blotting analysis, protein (8–30μg) was separated using a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-rad) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-rad). Membranes were incubated overnight at 4°C with the indicated antibodies: phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), p44/42 MAPK (Erk1/2), phospho-MEK1/2 (Ser217/221), MEK1/2, phospho-AKT (Ser473), AKT, phospho-p70S6K (Thr389), p70S6K, ATG5, cleaved-PARP (Cell Signaling), phospho-PDGFR-β (Tyr 857), PDGFR-β, Flk-1 (VEGFR2), GAPDH (Santa Cruz), phospho-VEGFR2 (Tyr1054/Tyr1059) (Thermo Fisher Scientific) and LC3B/MAP1LC3B (Novus Biologicals). For Odyssey CLx imaging, blots were incubated with secondary donkey anti-rabbit or donkey anti-mouse (IRDye 800CW) and donkey anti-goat, donkey anti-mouse, or donkey anti-rabbit (IRDye 680RD) (Li-Cor). For the protein array, the cell lysates were analyzed using the PathScan RTK Signaling Antibody Array Kit (Cell Signaling Technology), according to the manufacturer’s instructions.
Braggio D., Koller D., Jin F., Siva N., Zewdu A., Lopez G., Batte K., Casadei L., Welliver M., Strohecker A.M., Lev D, & Pollock R.E. (2019). AUTOPHAGY INHIBITION OVERCOMES SORAFENIB RESISTANCE IN S45F-MUTATED DESMOID TUMORS. Cancer, 125(15), 2693-2703.
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