The aCSF used for dissections and recordings contained (in mM) 127 NaCl, 26 NaHCO3, 10 glucose, 3 KCl, 2 CaCl, 1.25 NaH2PO4, and 1 MgCl2. Adenosine, 4-hydroxytamoxifen, and TFLLR were supplied by Sigma-Aldrich (Poole, UK); 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) was supplied by Abcam (Cambridge, UK); 14–22 amide, SKF 38393, and tetrodotoxin (TTX) were supplied by Tocris Bioscience (Bristol, UK). TFLLR, 14–22 amide, and SKF 38393 were dissolved in reverse-osmosis water; 4-hydroxytamoxifen was dissolved in corn oil; TTX was dissolved in citrate buffer; and Adenosine and DPCPX were dissolved in DMSO. The concentration of DMSO in working solutions did not exceed 0.1% (vol/vol).
14 22 amide
Manufactured by Bio-Techne
Sourced in United Kingdom, United States
The 14–22 amide is a laboratory product provided by Bio-Techne. It is a chemical compound used in various research applications. The core function of this product is to serve as a research tool, without further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine, by Merck Group (1 mentions)
Tetrodotoxin ttx, by Bio-Techne (1 mentions)
Skf38393, by Bio-Techne (1 mentions)
Tfllr, by Merck Group (1 mentions)
8 cyclopentyl 13 dipropyl xanthine dpcpx, by Abcam (1 mentions)
4 hydroxytamoxifen, by Merck Group (1 mentions)
Forskolin, by Bio-Techne (1 mentions)
Ici 118 551, by Merck Group (1 mentions)
Salmeterol, by Merck Group (1 mentions)
Tocrifluor t1117, by Bio-Techne (1 mentions)
O 1602, by Bio-Techne (1 mentions)
2 protocols using 14 22 amide
1
Electrophysiological Recording Conditions
2
Synthesis and Use of Fenoterol Analogs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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(R,R′)-Fenoterol [(R,R′)-Fen], (R,S′)-4′-methoxy-1-naphthylfenoterol [(R,S′)-MNF], and (R,R′)-MNF were synthesized as described previously28 (link). The following compounds from Tocris Bioscience (Ellisville, MO, USA) were used for cell treatments: O-1602 (GPR55 agonist), Tocrifluor T1117 (T1117, fluorescent GPR55 agonist), myristoylated protein kinase inhibitor-(14–22)-amide (PKI), and forskolin (adenylyl cyclase activator). Salmeterol (selective β2-AR agonist) and ICI-118,551 (ICI, selective β2-AR inverse agonist) were purchased from Sigma-Aldrich (St-Louis, MO, USA).
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