N 5 amino 1 carboxypentyl iminodiacetic acid ab nta

11-Amino-undecanethiol hydrochloride (99%), triethyleneglycol mono-11-mercaptoundecyl ether (95%; OEG-thiol) and 25% glutaraldehyde solution were purchased from Sigma Aldrich. Ethanol (HPLC grade) was purchased from Fisher Scientific Limited (Loughborough, UK). N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) was purchased from DoJindo Molecular Technologies. All aqueous solutions used in the preparation of NTA surfaces for protein attachment were prepared using ultrapure water (Elga LabWater Systems).

Poly(D,L-lactide-co-glycolic) acid (PLGA, 85:15, MW: 190,000–240,000) and fluorescein isothiocyanate isomer I were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (water-soluble carbodiimide, WSC), and N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) were from DOJINDO (Kumamoto, Japan). A purified mouse anti-BiP/GRP78 antibody (Cat#610979) was from BD Transduction Laboratories™ (Franklin Lakes, NJ, USA). Oleylamine and an anti-β-actin mouse monoclonal antibody were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). Anti-mouse immunoglobulin G (IgG), horseradish peroxidase (HRP)-linked (#7076) and anti-rabbit IgG, HRP-linked (#7074), anti-CHOP (#2895), anti-poly(ADP-ribose) polymerases (PARP) (#9542), anti-cleaved PARP (#5625), anti-cleaved cysteine aspartate-specific protease (caspase) 7 (#9491), anti-cleaved caspase 3 (#9661), anti-phospho-eIF2α (#3398), and anti-total eIF2α (#5324) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). An anti-iNOS mouse monoclonal antibody [37 (link)] and anti-SubAB anti-serum [38 (link)] were prepared as reported previously.