Cells were cultured on 8-well chamber slides, fixed in 3.7% formaldehyde, and permeabilized with 0.25% Triton X-100 in PBS. Labeling of the actin cytoskeleton was performed using phalloidin Alexa Fluor 564 (Invitrogen), and nuclei were marked using DAPI (4′, 6-diamidino-2-phenylindole) (Invitrogen) staining. Collagen was immunolabeled using rabbit anti-collagen type I (Chemicon) and anti-rabbit Alexa Fluor 488 secondary antibody (Invitrogen). Fluorescence imaging was performed using an LSM 510 META Axioplan 2 confocal laser scanning microscope equipped with a C-Apochromat 40x/1.2 water-immersion objective (Carl Zeiss). Alexa Fluor 488, Alexa Fluor 564, and DAPI were excited with a 488-nm argon laser, a 543-nm helium-neon laser, and a tunable (740-nm, two-photon) pulsed titanium sapphire laser (Verdi V10/Mira900), respectively. Image stacks were processed using Huygens Professional 2.4 (Scientific Volume Imaging) and Imaris 4.0 (Bitplane) software.
Lsm 510 meta axioplan 2 confocal laser scanning microscope
Manufactured by Zeiss
Sourced in Germany
The LSM 510 META Axioplan 2 is a confocal laser scanning microscope manufactured by Zeiss. It is capable of high-resolution imaging and analysis of biological samples. The device utilizes laser scanning technology to capture detailed images of specimens.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 564, by Thermo Fisher Scientific (1 mentions)
Rabbit anti collagen type 1, by Merck Group (1 mentions)
C apochromat 40x 1.2 water immersion objective, by Zeiss (1 mentions)
Alexa fluor 488 anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using lsm 510 meta axioplan 2 confocal laser scanning microscope
1
Multicolor Fluorescence Imaging of Cellular Cytoskeleton and Extracellular Matrix
2
Evaluating AgNPs Biofilm Integrity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The inhibitory effect of AgNPs on biofilm membrane integrity was evaluated using the Live/Dead Baclight bacterial viability kit (Molecular Probes, Invitrogen, Kit L13152) and confocal laser scanning microscopy (CLSM) using the modified protocol of Young et al. (2016) . Three K5 carriers were randomly chosen in each reactor before and after exposure to AgNPs.
The carriers were cut to expose the inner surfaces and were kept in 2 mL of bioreactor suspension that also contained suspended biomass, and placed in the special container for CLSM imaging. The biofilm and suspended-biomass containing samples were stained with two DNAbinding stains (SYTO-9 and propidium iodide). A minimum of 5 randomly-chosen microscopic fields were scanned for CLSM. All fluorescence images of biofilm were obtained using a LSM 510 META Axioplan 2 confocal laser scanning microscope with 40X objective (Carl Zeiss; Jena, Germany), equipped with 488 nm argon laser and 543 and 633 nm helium-neon lasers (Blanc et al., 2005) .
The carriers were cut to expose the inner surfaces and were kept in 2 mL of bioreactor suspension that also contained suspended biomass, and placed in the special container for CLSM imaging. The biofilm and suspended-biomass containing samples were stained with two DNAbinding stains (SYTO-9 and propidium iodide). A minimum of 5 randomly-chosen microscopic fields were scanned for CLSM. All fluorescence images of biofilm were obtained using a LSM 510 META Axioplan 2 confocal laser scanning microscope with 40X objective (Carl Zeiss; Jena, Germany), equipped with 488 nm argon laser and 543 and 633 nm helium-neon lasers (Blanc et al., 2005) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!