Phenotyping of conditioned monocyte-derived DCs (moDCs) and macrophages was performed by flow cytometry, anti-human CD14-fluorescein isothiocyanate (FITC), CD209/DC-SIGN-phycoerythrin (PE), CD1a-FITC, CD80-FITC CD86-PE, PD-L1-PE, CTLA-4-PE, CD163-PE, CD206-PE (BioLegend), HLA-DQ-FITC (BD Biosciences) were used.
Cd1a fitc
Manufactured by BioLegend
CD1a-FITC is a fluorescently-labeled antibody that binds to the CD1a surface antigen. CD1a is a glycoprotein that is expressed on the surface of dendritic cells, Langerhans cells, and some thymocytes. The FITC (fluorescein isothiocyanate) label allows for the detection and identification of CD1a-positive cells by flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Pd l1 pe, by BioLegend (2 mentions)
Cd163 pe, by BioLegend (2 mentions)
Cd206 pe, by BioLegend (1 mentions)
Ctla 4 pe, by BioLegend (1 mentions)
Hla dq fitc, by BD (1 mentions)
Cd86 pe, by BioLegend (1 mentions)
7 aminoactinomycin d, by Merck Group (1 mentions)
Cd80 fitc, by BioLegend (1 mentions)
Novocyte 2000 r flow cytometer, by Agilent Technologies (1 mentions)
Hla dr fitc, by BD (1 mentions)
2 protocols using cd1a fitc
1
Phenotyping of Monocyte-Derived Cells
2
Phenotyping of Myeloid Dendritic Cells
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Phenotyping of resting and TU-CM-moDCs was performed by flow cytometry using anti-human CD14-fluorescein isothiocyanate (FITC), CD209/DC-SIGN-phycoerythrin (PE), CD1a-FITC, CD1d-Peridinin-Chlorophyll-Protein (PerCP), CD80-FITC, CD86-PE, PD-L1-PE, CD163 –PE, CD206 –allophycocyanin (APC) (all from BioLegend), CTLA-4-PE (Sony Biotechnology Inc.), HLA-DR-FITC (BD Biosciences). Cell viability was assessed by 7-aminoactinomycin-D (7-AAD; 10 μg/ml; Sigma–Aldrich) staining. Samples were stained for 10 minutes with 7-AAD immediately before flow cytometric analysis. Fluorescence intensities were measured by Novocyte2000R Flow Cytometer (Agilent (Acea) Biosciences Inc., USA), and data were analyzed by the FlowJo v X.0.7 software (Tree Star).
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