Ltq mass spectrometry

Manufactured by Thermo Fisher Scientific

Sourced in United States

The LTQ mass spectrometry is a high-performance analytical instrument designed for the detection and identification of chemical compounds. It utilizes an ion trap mass analyzer to separate and detect ions based on their mass-to-charge ratio. The LTQ provides accurate and sensitive mass measurements, enabling the analysis of a wide range of molecules in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl pimelimidate, by Merck Group (2 mentions)

Close spelling variants of this product

LTQ) Orbitrap Velos Pro Mass Spectrometry LTQ FT mass spectrometry LTQ XL mass spectrometry LTQ-Orbitrap mass spectrometry LTQ Obitrap ETD mass spectrometry

2 protocols using ltq mass spectrometry

RNA Immunoprecipitation and Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

For RNA IPs, antibodies were covalently cross-linked to protein A Sepharose beads in dimethylpimelimidate (Sigma) at a 1:1 ratio of beads to antibody. Nuclear extract was rotated with antibody–cross-linked beads overnight at 4 ^oC. Half of the immunoprecipitates were used for SDS-polyacrylamide gel electrophoresis followed by western blot and half were used for RNAs extraction using phenol/chloroform extraction. For IP and mass spectrometry analysis, HeLa cells co-tranfected with tRNA and β-globin constructs were harvested 24 h after transfection and resuspended in 500 μl NET-2 buffer (50 mM Tris-HCl, pH 7.4/200 mM NaCl/0.1% NP40/1 mM PMSF) followed by sonication. After centrifuge, the supernatants were used for IPs using HA antibody covalently cross-linked to protein A Sepharose beads. The immunoprecipitates were used for liquid chromatography-tandem mass spectrometry analysis on a LTQ mass spectrometry (Thermo Fisher Scientific, USA).

Shi M., Zhang H., Wang L., Zhu C., Sheng K., Du Y., Wang K., Dias A., Chen S., Whitman M., Wang E., Reed R, & Cheng H. (2015). Premature termination codons are recognized in the nucleus in a reading-frame-dependent manner. Cell Discovery, 1, 15001-.

+ Open protocol

+ Expand

RNA Immunoprecipitation and Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

For RNA Immunoprecipitations, antibodies were covalently crosslinked to protein A Sepharose beads in dimethylpimelimidate (Sigma) at a 1:1 ratio of beads to antibody. Nuclear extract was rotated with antibody-crosslinked beads overnight at 4°C. Half of the immunoprecipitates were used for SDS-PAGE followed by western blot and half were used for RNAs extraction using phenol/chloroform extraction. For immunoprecipitation and mass spectrometry analysis, HeLa cells co-tranfected with tRNA and β-globin constructs were harvested 24 hrs after transfection and resuspended in 500 μl NET-2 buffer (50mM Tris-HCl, pH7.4/200mM NaCl/0.1% NP40/1mM PMSF) followed by sonication. After centrifuge, the supernatants were used for immunoprecipitations using HA antibody covalently crosslinked to protein A Sepharose beads. The immunoprecipitates were used for Liquid chromatography-Tandem mass spectrometry analysis (LC-MS/MS) on a LTQ mass spectrometry (Thermo Fisher Scientific).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!