The iPSCs or iPSC-derivatives were fixed in 10% formalin solution (Sigma-Aldrich #HT501128) for 15 min and permeabilized with 50 μg/mL digitonin (Sigma-Aldrich #D141) for 10 min at room temperature. Cells were blocked for 30 min at room temperature with a blocking solution made of DPBS with 1% Bovine Serum Albumin (BSA, Sigma-Aldrich #A7030) and 5% serum (Donkey Serum, Sigma-Aldrich #D9663; Goat Serum, ThermoFisher Scientific #31873). Cells were incubated overnight at 4 °C with 1% BSA solution containing a 1:200 dilution of primary antibodies. Cells were incubated for 30 min at room temperature with 1% BSA solution containing secondary antibodies at various concentrations (Table 3 ). Nuclei were counterstained with the Molecular Probes NucBlue Fixed Cell ReadyProbes Reagent (ThermoFisher Scientific #R37606).
D9663
Manufactured by Thermo Fisher Scientific
The D9663 is a laboratory instrument designed for scientific research and analysis. It is an essential piece of equipment for various applications in the scientific community. The core function of the D9663 is to perform precise measurements and data collection, enabling researchers to gather reliable information for their studies. This product is built with high-quality components to ensure accurate and consistent results.
Automatically generated - may contain errors
2 protocols using d9663
1
Immunocytochemistry of iPSCs and Derivatives
2
Immunocytochemistry of iPSCs and Derivatives
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The iPSCs or iPSC-derivatives were fixed in 10% formalin solution (Sigma-Aldrich #HT501128) for 15 min and permeabilized with 50 μg/mL digitonin (Sigma-Aldrich #D141) for 10 min at room temperature. Cells were blocked for 30 min at room temperature with a blocking solution made of DPBS with 1% Bovine Serum Albumin (BSA, Sigma-Aldrich #A7030) and 5% serum (Donkey Serum, Sigma-Aldrich #D9663; Goat Serum, ThermoFisher Scientific #31873). Cells were incubated overnight at 4 °C with 1% BSA solution containing a 1:200 dilution of primary antibodies. Cells were incubated for 30 min at room temperature with 1% BSA solution containing secondary antibodies at various concentrations (Table 3 ). Nuclei were counterstained with the Molecular Probes NucBlue Fixed Cell ReadyProbes Reagent (ThermoFisher Scientific #R37606).
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