Following treatment with 2 μg/mL MIF in the presence or absence of 50 μM 4-IPP for 24 h, primary joint capsule fibroblasts were fixed in 4% paraformaldehyde with 0.1% Triton X-100 (Sigma, St Louis, MO, USA) and subsequently incubated with 4% goat serum to block nonspecific binding. Then, cells were incubated with anti-TGF-β1 (Servicebio, Wuhan, China, 1:100), anti-MIF (Abcam, 1:100), or anti-CD74 (Santa Cruz, 1:50) primary antibodies overnight. Fluorescent secondary antibodies of FITC-labeled goat anti-rabbit IgG (Sigma, 1:400) and Cy3-labeled goat anti-rabbit IgG (Sigma, 1:400) were used to visualize the corresponding subsets. Cells were then stained with 4,6-diamidino-2-phenylindole (DAPI; Sigma, 1:4000) and phalloidin (Abcam, 1:1000), followed by observation under a confocal fluorescence microscope (Leica, Germany) and semi-quantitatively analyzed with Image-J software (National Institutes of Health, USA). The fluorescence was scored and quantified with blinding.
Anti tgf β1
Manufactured by Wuhan Servicebio Technology
Sourced in China
Anti-TGF-β1 is a protein that specifically binds to and neutralizes the transforming growth factor beta 1 (TGF-β1) cytokine. It is used for research purposes in cell and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
Fitc labeled goat anti rabbit igg, by Merck Group (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Confocal fluorescence microscope, by Leica (1 mentions)
Cy3 labeled goat anti rabbit igg, by Merck Group (1 mentions)
Anti cd74, by Santa Cruz Biotechnology (1 mentions)
Anti mif, by Abcam (1 mentions)
Confocal uorescence microscope, by Leica (1 mentions)
2 protocols using anti tgf β1
1
Immunofluorescence Analysis of Joint Fibroblast Responses
2
Immunofluorescence Analysis of TGF-β1 in Joint Fibroblasts
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Following treatment with 2 µg/mL MIF in the presence or absence of 50 µM 4-IPP for 24 h, primary joint capsule broblasts were xed in 4% paraformaldehyde with 0.1% Triton X-100 (Sigma, St Louis, MO, USA)
and subsequently incubated with 4% goat serum to block nonspeci c binding. Then, cells were incubated with anti-TGF-β1 (Servicebio, Wuhan, China, 1:100) primary antibodies overnight. Fluorescent secondary antibodies of FITC-labeled goat anti-rabbit IgG (Sigma, 1:400) were used to visualize the corresponding subsets. Cells were then stained with 4,6-diamidino-2-phenylindole (DAPI; Sigma, 1:4000) and phalloidin (Abcam, 1:1000), followed by observation under a confocal uorescence microscope (Leica, Germany).
and subsequently incubated with 4% goat serum to block nonspeci c binding. Then, cells were incubated with anti-TGF-β1 (Servicebio, Wuhan, China, 1:100) primary antibodies overnight. Fluorescent secondary antibodies of FITC-labeled goat anti-rabbit IgG (Sigma, 1:400) were used to visualize the corresponding subsets. Cells were then stained with 4,6-diamidino-2-phenylindole (DAPI; Sigma, 1:4000) and phalloidin (Abcam, 1:1000), followed by observation under a confocal uorescence microscope (Leica, Germany).
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