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4 diphenylacetoxy n methylpiperidine methiodide 4 damp

Manufactured by Cayman Chemical
Sourced in United States

4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) is a chemical compound used as a laboratory reagent. It functions as a muscarinic acetylcholine receptor antagonist.

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2 protocols using 4 diphenylacetoxy n methylpiperidine methiodide 4 damp

1

Intracerebral Pharmacological Modulation of Cognitive Processes

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Scopolamine (25 μg/kg, Tocris, UK), dissolved in 0.9% saline, was injected intraperitoneally (i.p.). Muscimol (1.25 μg/2 μl, Abcam, USA) dissolved in 0.9% saline was bilaterally injected into the mPFC 1 h prior to Scopolamine. M2-AChR antagonist methoctramine (MCT, GlpBio, USA), dissolved in 0.9% saline (0.5 μg/2 μl, 1 μg/2 μl or 2 μg/2 μl), was injected intracerebroventricularly (i.c.v.) (8 (link)). M3-AChR antagonist 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP, 100 pmol, Cayman, USA) was dissolved in 0.9% saline (1 μg/2 μl) and injected i.c.v (8 (link)). Rapamycin (Solarbio, China), dissolved in dimethyl sulfoxide (0.2 nmol/2 μl), was delivered i.c.v. 30 min prior to MCT injection. Groups of control animals for the above experiments received equal volumes of vehicle alone (0.9% saline or dimethyl sulfoxide). The experimental procedures are illustrated in Figure 1.
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2

Pharmacological Reagents for Nicotinic and Muscarinic Receptor Studies

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ACh, methyllycaconitine citrate (MLA), and 1-(4-methoxybenzyl)-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (BQCA) were purchased from Sigma-Aldrich (St. Louis, MO). (-)-Nicotine, mecamylamine hydrochloride, and tetrodotoxin (TTX) were obtained from Wako (Osaka, Japan). NS 9283, (±)-epibatidine, 4-(4-bromophenyl)-3a,4,5,9btetrahydro-3H-cyclopenta[c]quinolone-8-sulfonamide (4BP-TQS), scopolamine hydrobromide, and dihydro-βerythroidine hydrobromide (DHβE) were purchased from Tocris Bioscience (Bristol, UK). VU 0255035 was obtained from Abcam (Cambridge, UK). (+)-Muscarine iodide was purchased from Toronto Research Chemicals (Toronto, Canada). 4-Diphenylacetoxy-N-methyl-piperidine methiodide (4-DAMP) was obtained from Cayman Chemical (Ann Arbor, MI). ACh, MLA, (-)-Nicotine, mecamylamine hydrochloride, DHβE, (+)-muscarine iodide, scopolamine hydrobromide, and TTX were dissolved in distilled water (Thermo Fisher Scientific, Waltham, MA). 4BP-TQS, NS 9283, (±)-epibatidine, BQCA, VU 0255035, and 4-DAMP were dissolved in DMSO (Wako).
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