Spleen fragments from control or VS-4718–treated mice (50 mg/kg) were frozen in RNALater and lysed in mPER buffer (ThermoFisher Scientific) with 3× protease/phosphatase inhibitors (Halt Protease and Phosphatase Inhibitor Cocktail). For lysates from RetroNectin assays, pre-B cells adhering to RetroNectin monolayers were lysed by addition of mPER buffer directly to the well. Western blotting was performed using anti- p-P130Cas and anti-pFAK-Y397 antibodies (Cell Signaling Technologies, 4015 and 3283, respectively). ELISA was done using the FAK [pY397] Phospho-ELISA Kit (ThermoFisher Scientific, KHO0441).
Anti p p130cas
Manufactured by Cell Signaling Technology
Anti-p-P130Cas is an antibody that detects the phosphorylated form of the P130Cas protein. P130Cas is an adaptor protein involved in various cellular signaling pathways. The antibody can be used to study the phosphorylation status of P130Cas in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
M per buffer, by Thermo Fisher Scientific (1 mentions)
Anti pfak y397, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Pall Corporation (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti lats1, by Proteintech (1 mentions)
Anti n cadherin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti β catenin, by Cell Signaling Technology (1 mentions)
Anti pak, by Cell Signaling Technology (1 mentions)
Anti limk, by Cell Signaling Technology (1 mentions)
Anti yap, by Proteintech (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Anti cortactin, by Cell Signaling Technology (1 mentions)
2 protocols using anti p p130cas
1
Signaling Pathway Analysis in Spleen Cells
2
Western Blot of Cell Signaling Proteins
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Protein from cell or tissue lysates was separated by SDS-polyacrylamide gel electrophoresis (PAGE) and electro-transferred onto a polyvinylidene difluoride (PVDF) membrane (Pall Corp, Port Washington, NY). Then the membranes were blocked with 5% skimmed milk and incubated using primary antibodies anti-CSRP2 (1:800 dilution, Sigma), anti-GAPDH, anti-p130Cas, anti-p-p130Cas (Tyr410), anti-PAK, anti-p-PAK, anti-LIMK, anti-p-LIMK, anti-cortactin, anti-p-cortactin, anti-E-cadherin, anti-N-cadherin, anti-vimentin, anti-β-catenin (Cell Signaling Technology, Beverly, MA), anti-LATS1, anti-Yap (Proteintech), anti-ERK1/2, anti-p-ERK1/2 (Thr202/Tyr204), anti-p-Yap (Ser127) (Absci), anti-p-LATS1 (Thr1079/1041) (Bioss) at 4 °C. Then the membranes were incubated with appropriate secondary antibodies (anti-rabbit IgG, 1:3000 dilution, #7074; cell signaling). Enhanced chemiluminescence (Pierce, Rockford, 1L, USA) was used to detect the signal.
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