ALDH−, ALDH+, and CD44+/CD24− cells were first treated with vehicle control (DMSO) or treatment (ASR490, DAPT, MG132, or CQ) for prescribed doses and time points. Cell viability assays: Alamar blue (Life Technologies Corporation Eugene, OR) and EdU Cell Proliferation (using the EdU-Click 488 kit, cat# BCK-EdU488-1, Sigma), were then performed per the manufacturer’s instructions.
Edu click 488 kit
Manufactured by Merck Group
The EdU-Click 488 kit is a laboratory product designed for the detection and quantification of cellular proliferation. It utilizes the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) into newly synthesized DNA, which can then be detected using a fluorescent dye. The kit provides a straightforward and sensitive method for analyzing cell division and proliferation in various experimental settings.
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Lab products found in correlation
2 protocols using edu click 488 kit
1
ALDH+, CD44+/CD24- Cell Viability
2
Inflammasome Activation and Mitochondrial DNA Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells adhered to covers pre-coated with poly-L-lysine were stimulated with inflammasome activators, followed by fixation with 4% paraformaldehyde for 10 min at room temperature. Cells were then washed twice with PBS and permeabilized with PBS containing 1% Triton-X-100 for 10 min, followed by washing with PBS for twice and blockage in 10% normal goat serum at 37 C for 30 min. Cells were incubated with primary antibody for 2 h, followed by washing with PBS for three times and further incubation with fluorescenceconjugated secondary antibody for 1 h. Nuclei were stained with DAPI. For EdU staining, WT BMDM cells were stimulated with 200 ng/ml LPS and 10 mM EdU for 3 h to specifically label the mitochondrial DNA as previously reported. 35 (link) A EdU-Click 488 kit (Sigma-Aldrich) was used to visualize EdU inside cells. Cells were visualized through an UltraVIEW VoX imaging system (PerkinElmer).
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