Horseradish peroxidase conjugated anti rabbit igg and mouse igg

Kits for detecting the Malondialdehyde (MDA) level, activity of manganese superoxide dismutase (Mn-SOD), Acetylcholine (Ach) level, activity of Acetylcholinesterase (AChE), and activity of Choline acetyltransferase (ChAT) were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Primary antibodies, Postsynapticdensity 95 (PSD95), BDNF, Cleaved Caspase-1, Bcl-2, Bax and Cleaved Caspase-3, were obtained from Abcam, Inc. (Cambridge, UK). Secondary antibodies (horseradish peroxidase-conjugated anti-rabbit IgG and mouse IgG) and NLRP3 were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Ligustilide were purchased from Chengdu Herbpurifed Co., Ltd. (purity > 98%, molecular weight: 190.24 g/mol, Figure 1A). The Malondialdehyde (MDA) assay kit and Mn-SOD and Cellular Glutathione Peroxidase Assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies mainly include Postsynapticdensity 95 (PSD95), Postsynapticdensity 93 (PSD93), NLRP3, NF-κB, IL-1β, IL-10, Bax, Bcl-2 and Caspase-3 were purchased from Cell Signaling Technology, Inc. Anti-β-actin was obtained from Sigma-Aldrich. All secondary antibodies (horseradish peroxidase-conjugated anti-rabbit IgG and mouse IgG) were purchased from Cell Signaling Technology, Inc. Other reagents were the highest quality commercial reagents.