Tris was purchased from Carl Roth (Karlsruhe, Germany), isopropyl β-d -1-thiogalactopyranoside (IPTG) from Serva (Heidelberg, Germany), methacrylonitrile from Fluka (Buchs, Switzerland) and CoCl2 and FeSO4*7H2O from Merck. HPLCMS grade acetonitrile was purchased from J.T.Baker/Avantor Performance Materials (Deventer, The Netherlands). All other chemicals were obtained from Sigma–Aldrich (St. Luis, MO, USA) and used without further purification.
E. coli cells were cultivated in an RS 306 shaker (Infors, Bottmingen, Switzerland), a Multitron shaker (Infors AG Bottmingen, Switzerland) and a Certomat BS-1, and the cells were harvested with an Avanti J-20 XP centrifuge (Beckman Coulter, Brea, CA, USA). Cell pellets were disrupted by a 102C converter with a Sonifier 250 (Branson, Danbury, CT, USA), and the cell-free extract was obtained by centrifugation in an Avanti J-20 XP centrifuge (Beckman Coulter). Reactions were performed on a Thermomixer comfort (Eppendorf, Hamburg, Germany). HPLC/MS analysis was carried out on an Agilent Technologies (Santa Clara, CA, USA) 1200 Series equipped with G1379B degasser, G1312B binary pump SL, G1367C HiP-ALS SL autosampler, a G1314C VWD SL UV detector, G1316B TCC SL column oven and a G1956B MSD. A positive electrospray ionization mode was used as ionization method.
E. coli cells were cultivated in an RS 306 shaker (Infors, Bottmingen, Switzerland), a Multitron shaker (Infors AG Bottmingen, Switzerland) and a Certomat BS-1, and the cells were harvested with an Avanti J-20 XP centrifuge (Beckman Coulter, Brea, CA, USA). Cell pellets were disrupted by a 102C converter with a Sonifier 250 (Branson, Danbury, CT, USA), and the cell-free extract was obtained by centrifugation in an Avanti J-20 XP centrifuge (Beckman Coulter). Reactions were performed on a Thermomixer comfort (Eppendorf, Hamburg, Germany). HPLC/MS analysis was carried out on an Agilent Technologies (Santa Clara, CA, USA) 1200 Series equipped with G1379B degasser, G1312B binary pump SL, G1367C HiP-ALS SL autosampler, a G1314C VWD SL UV detector, G1316B TCC SL column oven and a G1956B MSD. A positive electrospray ionization mode was used as ionization method.