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Primary antibodies against hdac2

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Primary antibodies against HDAC2 are laboratory reagents used to detect and study the presence and distribution of the HDAC2 protein in biological samples. HDAC2 is a histone deacetylase enzyme involved in the regulation of gene expression.

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Lab products found in correlation

Dld 1, by American Type Culture Collection (2 mentions) Hct116, by American Type Culture Collection (2 mentions) Sw480, by American Type Culture Collection (2 mentions) Sw620, by American Type Culture Collection (2 mentions)

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HDAC2 (67 citations)

2 protocols using primary antibodies against hdac2

1

IHC Analysis of HDAC2 in CRC Cell Lines

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Commercially available tissue microarray (TMA) slides (HLin-Ade075Met-01, Shanghai Biochip Co., Ltd., Shanghai, China) containing histologically con rmed tissues from CRC patients were purchased for immunohistochemistry (IHC) analysis. Speci c primary antibodies against HDAC2 (Cell Signaling Technology) were used for IHC with a 2-step protocol.
Cell culture DLD1, HCT116, SW480 and SW620 cells were obtained from ATCC. DLD1 and HCT116 cells were cultured in RPMI 1640 medium, while SW480 and SW620 were cultured in high glucose DMEM. All media were supplemented with 10% FBS, 100 µg/m L penicillin and 100 U/m L streptomycin. The DLD1 HDAC2 KO cell line was constructed in the lab of Professor Run-lei Du, who is our collaborator in this study 25 .
Hu X., Xing W., Zhao R., Tan Y., Wu X., Ao L., Li Z., Yao M., Yuan M., Guo W., Li S., Yu J., Ao X., & xu x. (2020). HDAC2 inhibits EMT-mediated cancer metastasis by downregulating the long noncoding RNA H19 in colorectal cancer.
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2

Immunohistochemical Analysis of HDAC2 in CRC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available tissue microarray (TMA) slides (HLin-Ade075Met-01, Shanghai Biochip Co., Ltd., Shanghai, China) containing histologically con rmed tissues from CRC patients were purchased for immunohistochemistry (IHC) analysis. Speci c primary antibodies against HDAC2 (Cell Signaling Technology) were used for IHC with a 2-step protocol.
Cell culture DLD1, HCT116, SW480 and SW620 cells were obtained from ATCC. DLD1 and HCT116 cells were cultured in RPMI 1640 medium, while SW480 and SW620 were cultured in high glucose DMEM. All media were supplemented with 10% FBS, 100 µg/m L penicillin and 100 U/m L streptomycin. The DLD1HDAC2 KO cell line was constructed in the lab of Professor Run-lei Du, who is our collaborator in this study 25 .
Hu X., Xing W., Zhao R., Tan Y., Wu X., Ao X., Ao L., Li Z., Yao M., Yuan M., Guo W., Li S., Yu J., & Xu X. (2020). HDAC2 Inhibits EMT-Mediated Cancer Metastasis by Downregulating the Long Noncoding RNA H19 in Colorectal Cancer.
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