Tyrosinase tyr from mushroom

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Sourced in United States

Tyrosinase (TYR) from mushroom is an enzyme that catalyzes the oxidation of tyrosine to dopaquinone, a key step in the melanin biosynthesis pathway. It is extracted and purified from mushrooms. The core function of Tyrosinase (TYR) is to catalyze the conversion of tyrosine to dopaquinone in in-vitro experiments.

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Mushroom tyrosinase (203 citations) Tyrosinase (112 citations) Tyrosinase from mushroom (35 citations) Mushroom TYR (6 citations)

3 protocols using tyrosinase tyr from mushroom

Enzymatic Assays for Polyphenol Oxidation

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Lipase B from Candida antarctica (CALB) solution (Lipozyme CALB),
lipase from Thermomyces lanuginosus (TLL) solution (Lipozyme TL 100L), catalase from Aspergillus niger (CAT) solution (Catazyme), and
glucose oxidase (Gluzyme Mono 10.000 BG) (GOX) were purchased from
Novozymes (Copenhagen, Denmark). Genetically modified lipase from
((Geo)Bacillus thermocatenulatus without
native cysteines (C65S/C296S) BTL) was produced by Dr. de las Rivas
and purified following the previous report (obtaining a solution of
2.5 mg lipase/mL by Bradford assay determination).^{37 (link)} Copper(II) sulfate pentahydrate [Cu₂SO₄·5H₂O] and hydrogen peroxide (33%) were from Panreac
(Barcelona, Spain). p-Aminophenol (pNP), sodium phosphate, sodium acetate, tyrosinase (TYR) from mushroom,
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium
salt (ABTS), benzoquinone, and hydroquinone were purchased from Sigma-Aldrich
(St. Louis, MO). 3,4-Dihydroxy-l-phenylalanine (l-DOPA) was from Alfa-Aesar (MA, EEUU). 3,4-Dihydroxy-l-phenylalanine
methyl ester hydrochloride (l-DOPA methyl ester (DOPAME))
was purchased from Carbosynth (Berkshire, U.K.). Horseradish peroxidase
(HRP) was from Thermo Scientific (Madrid, Spain).

Losada-Garcia N., Jimenez-Alesanco A., Velazquez-Campoy A., Abian O, & Palomo J.M. (2021). Enzyme/Nanocopper Hybrid Nanozymes: Modulating Enzyme-like Activity by the Protein Structure for Biosensing and Tumor Catalytic Therapy. ACS Applied Materials & Interfaces, 13(4), 5111-5124.

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Lipid Deposition and Enzymatic Assay

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Arachidic acid (AA, 312,53 g mol -1 ), 1,2-dipalmitoylsn-glycero-3-phospho-(1'-rac-glycerol) (DPPG, 744.95 g mol -1 ) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, 734,08 g mol -1 ) were purchased from Avanti Polar Lipids, while tyrosinase (Tyr, from mushroom, activity of 5370 U mg -1 ) was purchased from Sigma-Aldrich. Phosphate buffered saline (PBS) solution was prepared using NaH 2 PO 4 (119.98 g mol -1 ) and Na 2 HPO 4 .2H 2 O (117,98 g mol -1 ) from Sigma-Aldrich. NaCl (Sigma Aldrich, 58.44 g mol -1 ) was used as a supporting electrolyte in electrochemical measurements. To Langmuir films experients, the lipid solutions were prepared in chloroform (Sigma-Aldrich), and PBS prepared with ultrapure water (resistivity of 18.2 MΩ cm). All chemical reagents were used without further purification.

Pereira M.S., Maximino M.D., Martin C.S., Aoki P.H.B., Oliveira ON J.r, & Alessio P. (2021). Lipid-matrix effects on tyrosinase immobilization in Langmuir and Langmuir-Blodgett films. Anais da Academia Brasileira de Ciencias, 93(1).

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Enzymatic Reactions with Glucose Oxidase and Tyrosinase

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All chemicals and solvents were purchased from SigmaeAldrich. Solvents were of reagent grade and used as supplied. Deionized water was obtained from a Millipore purifier, with a resistivity of 18.2 MU/cm.
Glucose oxidase (GOX), from Aspergillus niger, type VII (activity plus 0.001 kat/mg), and Tyrosinase (Tyr), from Mushroom (activity of 6.10 À5 kat/mg), were purchased from Sigma Chemical Co. (USA).

Medina-Plaza C., García-Hernández C., de Saja J.A., Fernández-Escudero J.A., Barajas E., Medrano G., García-Cabezón C., Martin-Pedrosa F, & Rodriguez-Mendez M.L. (2015). The advantages of disposable screen-printed biosensors in a bioelectronic tongue for the analysis of grapes. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 62(2).

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