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Cd57 pe

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CD57-PE is a laboratory reagent used for the detection and analysis of CD57-positive cells in biological samples. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the CD57 antigen, which is expressed on a subset of natural killer cells and a small population of T cells. The CD57-PE reagent is designed for use in flow cytometry applications to identify and quantify CD57-positive cell populations within a sample.

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Lab products found in correlation

Cd25 pe cy7, by BD (1 mentions) Lag3 pe, by BD (1 mentions) Ctla4 apc, by BD (1 mentions) Dnam bb515, by BD (1 mentions) Cd45ro pe cy7, by BD (1 mentions) Ccr7 pe, by R&D Systems (1 mentions) Cd45 apc h7, by BD (1 mentions) Icos pe cy7, by Thermo Fisher Scientific (1 mentions) Cd8 bv510, by BD (1 mentions) Pd1 fitc, by BD (1 mentions) Nkg2d pe cy7, by BD (1 mentions) Cxcr3 af488, by BD (1 mentions) Hla dr bb515, by BD (1 mentions) Cd161 pe, by BD (1 mentions) Nkp46 pe cy7, by BD (1 mentions) Nkg2a apc, by R&D Systems (1 mentions) Cxcr4 apc, by BD (1 mentions) Cd56 bv421, by BD (1 mentions) Tcrγδ apc, by BD (1 mentions) Cd3 percp cy5, by BD (1 mentions)

2 protocols using cd57 pe

1

Comprehensive Flow Cytometry Immunophenotyping

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Different immune cell subpopulations were immunophenotyped with flow cytometry from fresh PB samples with 6 panels of different cell-surface markers, including the following immune checkpoint receptors and cytotoxicity and migration markers: CD3-PerCP-Cy5.5 (BD, catalog 332771), CD4-PE-Cy7 (BD, catalog 560649), CD45-APC-H7 (BD, catalog 560178), CD8-BV510 (BD, 563919), CD56-BV421 (BD, 562751), CXCR1-FITC (BioLegend, catalog 341606), CD16-PE (BD, 561313), TCR γδ-APC (BD, catalog 555718), PD1-FITC (BD, catalog 557860), LAG3-PE (BD, catalog 125209), ICOS-PE-Cy7 (eBioscience, catalog 25-9948-42), CTLA-4–APC (BD, catalog 560938), HLA-DR-BB515 (BD, catalog 560938), CD27-PE (BD, catalog 555441), CD25-PE-Cy7 (BD, catalog 561405), CD11b-APC (BD, catalog 550019), NKG2C-AF488 (R&D Systems, catalog FAB138G), CD161-PE (BD, catalog 556081), NKG2D-PE-Cy7 (BD, catalog 562365), NKG2A-APC (R&D Systems, catalog FAB1059A), DNAM-BB515 (BD, catalog 565152), CD57-PE (BD, catalog 560844), NKp46-PE-Cy7 (BD, catalog 562101), NKp30-AF647 (BD, 558408), CXCR3-AF488 (BD, catalog 561730), CCR7-PE (R&D Systems, catalog FAB197P), CD45RO-PE-Cy7 (BD, catalog 560608), and CXCR4-APC (BD, catalog 560936). CD45+ lymphocytes were acquired with the BD FACS Verse, and the data were analyzed with FlowJo, version 10.4. The results are shown in Supplemental Table 1.
Huuhtanen J., Kasanen H., Peltola K., Lönnberg T., Glumoff V., Brück O., Dufva O., Peltonen K., Vikkula J., Jokinen E., Ilander M., Lee M.H., Mäkelä S., Nyakas M., Li B., Hernberg M., Bono P., Lähdesmäki H., Kreutzman A, & Mustjoki S. (2023). Single-cell characterization of anti–LAG-3 and anti–PD-1 combination treatment in patients with melanoma. The Journal of Clinical Investigation, 133(6), e164809.
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2

Immunophenotyping of NK and Senescent T-cells in Early MS

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Peripheral blood mononuclear cells (PBMC) were isolated from blood samples taken in EDTA collection tubes using Lymphoprep, and subsequently cryopreserved in fetal calf serum with 10% DMSO. The gating strategy of the study is shown in Figure 1. NK-cell immunophenotype was evaluated in early MS patients (n=120) as previously reported [12] , using the following conjugated monoclonal antibodies: anti-CD3-PerCP, anti-CD56-APC (BD Biosciences), NKG2C-PE (R&D Systems), and DAPI. A selected panel of immunological markers of differentiated /senescent T-cells previously related to CMV infection (CD27-CD28-/CD57+/LILRB1+ T-cells) [16] [17] [18] were evaluated in a subcohort of MS patients (early MS, n=35; non-early MS, n=32) and controls (n=32) stratified for CMV serology. Samples were stained by indirect immunofluorescence with LILRB1 HP-F1and anti-mouse Ig-PE-Cy7 (Biolegend), washed and further incubated with anti-CD3-APC-H7, anti-CD27-PERCP-Cy5.5, anti-CD28-PE-CF594, CD57-PE (BD Biosciences), and anti-CD4-FITC (eBioscience), anti-CD8-BV510 (Biolegend). All samples were analyzed at the Flow Cytometry Unit (UPF/CRG, Barcelona) with an LSRII-Fortessa flow cytometer (BD Biosciences).
Alari-Pahissa E., Moreira A., Zabalza A., Alvarez-Lafuente R., Munteis E., Vera A., Arroyo R., Alvarez-Cermeño J.C., Villar L.M., López-Botet M, & Martínez-Rodríguez J.E. (2018). Low cytomegalovirus seroprevalence in early multiple sclerosis: a case for the 'hygiene hypothesis'?. European journal of neurology, 25(7).
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