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Nylon syringe filter

Manufactured by PerkinElmer
Sourced in China

The Nylon syringe filter is a laboratory equipment designed to filter liquids. It features a nylon membrane that removes particulates from the sample, ensuring a clear and contaminant-free solution for further analysis or processing.

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2 protocols using nylon syringe filter

1

Quantitative Analysis of Pesticides using LC-MS/MS

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LC System—QSight® LX-50 LC (PerkinElmer, Shelton, CT).

MS system——QSight™ 420 MS/MS detector with ESI and APCI source with HSID interface and Simplicity 3Q™ software platform (PerkinElmer, Shelton, CT).

LC Column—Quasar SP Pesticides C18, 100 mm long, 4.6 mm ID, 2.7 μm particle size, and particle is superficially porous (PerkinElmer, Shelton, CT).

Mixer—analog vortex mixer (VWR, Radnor, PA).

Centrifuge—Eppendorf centrifuge 5430 (Eppendorf Co. Ltd).

Polypropylene centrifuge tubes—15 mL and 50 mL (PerkinElmer, Shelton CT).

Glass volumetric flasks—50 mL (VWR, Radnor, PA).

Syringes—3-mL plastic syringes with Luer lock (Becton Dickinson, Fingerlakes, NJ).

Filter—Nylon syringe filter, diameter 30 mm, 0.22 μm pore size (PerkinElmer, Shelton, CT).

LC vials—2-mL amber glass (PerkinElmer, Shelton, CT).

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2

Quantifying Epicatechin in Freeze-Dried Samples

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Epicatechin was determined following the method used by Guetsky et al. (2005) , with some modifications. Freeze-dried samples (20 mg) were homogenised in methanol: water (1:1; v/v) solution. Thereafter, the Eppendorf tubes were centrifuged for 10 min at 14 000 x g. The supernatant was transferred to the new tubes, and subsequently filtered through a 0.45 μm membrane (Nylon syringe filter, PerkinElmer™, China) prior to injection. The analyses were carried out using a Flexar™ HPLC system (PerkinElmer, USA) consisting of a Flexar Isocratic LC Pump Platform and a variable wavelength Flexar UV/ ViS LC detector. Separation was done on an Analytical C18 column (100 x 4.6 mm; 5 μm) at 25 °C, using water/methanol (25:75 v/v) as a mobile phase, with a flow rate of 1 ml min -1 . Chromatographic peak of the epicatechin was identified by comparing the retention time with that of the pure epicatechin (HPLC grade) standard at 320 nm.
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