37 c cell incubator

The instruments used included a 0.15 M² freeze dryer (Advantage Plus, VirTis, ID, 815 Route 208 Gardiner, New York, NY, USA), a DSC thermal analyzer (PerkinElmer, Waltham, MA, USA), a Scaning Electron Microscope(SEM) (FEI, Hillsboro, OR, USA), and 37 °C cell incubator (Thermo, 81 Wyman Street, Waltham, MA, USA).

NSCLC cells H460, H1299, A549, PC-9, and H1975 were purchased from the ATCC cell bank. The NSCLC cells were cultured in RPMI medium 1640 Basic (GIBCO, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Longsera, Shanghai, China) and 1% penicillin/streptomycin (NCM BioTECH, Suzhou, China). Cells were cultured in a 37 °C cell incubator (Thermo Fisher Scientific, Waltham, MA, USA) with 5% CO₂. When the cells reached approximately 80% plate bottom coverage, they were incubated in LTP-treated medium for 0 s, 10 s, 15 s, 20 s, 25 s, and 30 s.

HEK293T cells (CRL-11268, ATCC) were cultured with Dulbecco’s modified Eagle’s medium (Gibco) supplied with 10% (v/v) fetal bovine serum (Gibco) and penicillin–streptomycin (50–50 μg/ml) and placed in a 37 °C cell incubator (Thermo) supplied with 5% CO₂. Cortical neurons were dissociated from newborn pups of Kunming mice (obtained from the Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei) as described previously [25 (link)]. Briefly, the mouse cerebral cortex was dissected and digested with 0.25% trypsin–EDTA (Gibco) at 37 °C for 12 min and then plated on glass coverslips coated with poly-L-lysine (Sigma) at a density of 80,000 cells per 8 mm × 8 mm. Cells were cultured at 37 °C with 5% CO₂ in MEM medium with the addition of 2% (v/v) B27 (Gibco), 0.5% (w/v) glucose (Sigma), 100 mg/l transferrin (Sigma), 5% (v/v) fetal bovine serum (Gibco), and 2 mM Ara-C (Sigma).