Pp60 ti

No yeast-containing doughs were covered with parafilm paper immediately after kneading and loaded into the rheometer at the earliest. Before conducting any rheological measurement, doughs were allowed to rest in the measurement position for 10 min as equilibration time, i.e., the necessary time to allow the stresses induced during sample loading to relax. The required equilibration time was selected according to previous time sweep tests carried out within the linear region at 1 Hz and 25 °C during 30 min. The time sweep test showed that in approximately 10 min, the values of G' and G'' did not vary significantly with time (supplementary material 2). After adjustment of the gap, the exposed edges of the dough samples were always covered with vaseline oil (Panreac Quimica S.A., Castellar del Valles, Spain) to avoid sample drying during measurements. All rheological tests were run in duplicate in a controlled stress rheometer (Haake RheoStress 1, Thermo Fischer Scientific, Scheverte, Germany) with a titanium parallel plate geometry sensor PP60 Ti (60 mm diameter and 3 mm gap).

Before conducting any rheological measurement, doughs were allowed to rest in the measurement position for 10 min as equilibration time, i.e., the necessary time to allow the stresses induced during sample loading to relax. The required equilibration time was selected according to previous time sweep tests carried out within the linear region (1 Pa) at 1 Hz and 25°C during 30 min. The time sweep test showed that in less than 10 min values of G' and G'' became independent of time. After adjustment of the gap, the excess dough was removed and the exposed edges of the samples were always covered with vaseline oil (Panreac Química S.A., Castellar del Valles, Spain) to avoid sample drying during measurements. In this study, yeast-containing doughs were analyzed after kneading (0min of fermentation), 45 and at 90 min of fermentation in order to include the effects of the gas volume and fermentation metabolites. All rheological tests were run in duplicate in a controlled stress a rheometer (Haake RheoStress 1, Thermo Fischer Scientific, Scheverte, Germany) with a titanium parallel plate geometry sensor PP60 Ti (60 mm diameter, and 3 mm gap).