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The CNE-2 is a laboratory centrifuge designed for high-speed separation of biological samples. It can achieve centrifugal forces up to 20,000 x g, making it suitable for a wide range of applications, including the sedimentation of cells, organelles, and macromolecules. The CNE-2 features a user-friendly interface, programmable speed and time settings, and safety mechanisms to ensure reliable and efficient sample processing.

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2 protocols using cne 2

1

Nasopharyngeal Carcinoma Cell Line Culture

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Three nasopharyngeal carcinoma cell lines (CNE-1, CNE-2, and SUNE-1) were obtained from the Shanghai Institute of Cell Biology (Shanghai, China). CNE-1, CNE-2, and SUNE-1 cell lines were cultured in RPMI 1640 (Gibco BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences, Logan, UT, USA) at 37°C with humidified 5% CO2.
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2

Nasopharyngeal Cell Line Culture

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Four NPC cell lines, i.e., CNE1, CNE2, SUNE1, and HONE1, along with the human immortalized nasopharyngeal epithelial cell line NP69 were obtained from the Shanghai Institute of Cell Biology (Shanghai, China). Every new batch of cell lines underwent mycoplasma testing and analysis of short tandem repeats. All the cell lines were incubated in the RPMI 1640 medium (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) that contained 10% Fetal Bovine Serum (FBS, Gibco, Australia), penicillin, and streptomycin (100 U/mL, each) at the optimal temperature of 37 °C and with 5% CO2.
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