1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3phosphoglycerol (DOPG) and 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (18:1 CL) were purchased from Avanti Polar Lipids as chloroform solutions.
1 3 bis 1 2 dioleoyl sn glycero 3 phospho glycerol
Manufactured by Avanti Polar Lipids
1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol is a phospholipid compound. It is used as a laboratory reagent.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
1 2 dioleoyl sn glycero 3 phosphoglycerol, by Avanti Polar Lipids (1 mentions)
Sinapinic acid, by Merck Group (1 mentions)
1 octadecanethiol, by Merck Group (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Sodium l ascorbate, by Merck Group (1 mentions)
3 mercaptopropionic acid, by Merck Group (1 mentions)
6 mercaptohexanol, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Protein assay dye reagent concentrate, by Bio-Rad (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n biotinyl polyethylene glycol 2000 dspe peg biotin, by Avanti Polar Lipids (1 mentions)
Octylglucoside, by Anatrace (1 mentions)
Micro bicinchoninic acid assay, by Thermo Fisher Scientific (1 mentions)
3 3 cholamidopropyl dimethylammonio 1 propane sulfonate, by Anatrace (1 mentions)
Imperial protein stain solution, by Thermo Fisher Scientific (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions)
5 protocols using 1 3 bis 1 2 dioleoyl sn glycero 3 phospho glycerol
1
Lipid Preparation for Biophysical Studies
2
Biophysical Analysis of Cytochrome c Interactions
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Cyt c (from equine heart), phosphatidylcholine (eggPC, from egg yolk), 3-Mercaptopropionic acid, 6-Mercaptohexanol, potassium chloride (KCl), sodium l -ascorbate, ethanol, 1-Octadecanethiol (ODT), trifluoroacetic acid (TFA), acetonitrile (ACN), and sinapinic acid were purchased from Sigma (St. Louis, MO). The lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (18:1, CL) were obtained from Avanti Polar Lipids (Alabaster, AL). Sodium dodecyl sulfate (SDS) was purchased from Merck (Darmstadt, Germany). Deuterium oxide (D2O, 99.9%) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA). Immobilized pepsin on 6% agarose beads was purchased from Thermo Scientific (Waltham, MA). A protein assay dye reagent concentrate was purchased from Bio-Rad (Hercules, CA). All aqueous solutions were prepared using ultrapure water (18.2 MΩ cm) from a Milli-Q purification system (Millipore, Burlington, MA). A CL binding dye, 10-nonyl acridine orange (NAO), was obtained from Enzo Life Sciences (Farmingdale, NY).
3
Lipid Monolayer Formation Using LBT
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Only commercially available resources were used for this study, available as detailed in the key resources table . Phosphate buffered saline (PBS) and other inorganic salts were obtained at p.A. quality (VWR). The lipids used were 2,3-dimethoxy-5-methyl-6-(farnesylfarnesyl)-1,4-benzo quinone (Q6, neat oil, extracted from Saccharomyces cerevisiae), purchased from Avanti Lipids (via Merck), Decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl]-5,6-dimethoxy3-methylcyclohexa-2,5-diene-1,4-dione (Q10), purchased from Avanti Lipids (via Merck), 1,2-Distearoyl-sn-glycero-3lipid mass concentrations of 0.1 mg/ml for Q6 and 0.25 mg/ml for DSPE. In addition Ethanol≤ ≥ ≥99.9% pure from VWR and≥ phosphoethanolamine (DSPE) as well as 1’,3’-bis[1,2-dioleoyl-sn-glycero-3-phospho] glycerol (sodium salt) (18:1, cardiolipin) were purchased from Avanti Polar Lipids. The lipids were dissolved in pure chloroform from Carl Roth (assay: 99.9%), with.
Milli-Q water (Milli-pore, TOC value 2 ppb, resistivity 18 MΩ) were used throughout for making solutions and for cleaning equipment. The defined volumes of lipid containing solutions of Q6 and DSPE, respectively, were dropped onto the water surface of an LBT (R & K, Potsdam, Germany) successively.
Milli-Q water (Milli-pore, TOC value 2 ppb, resistivity 18 MΩ) were used throughout for making solutions and for cleaning equipment. The defined volumes of lipid containing solutions of Q6 and DSPE, respectively, were dropped onto the water surface of an LBT (R & K, Potsdam, Germany) successively.
4
Synthetic Lipid Composition for Membrane Analysis
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DOPC, DOPE, DOPG, 1’,3’-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (18:1 CL), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000 (DSPE-PEG-biotin) were from Avanti Polar Lipids. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE-Texas Red) was from Invitrogen.
5
Optimized Membrane Protein Purification and Labeling
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All lipids were purchased from Avanti Polar Lipids: L-α-phosphatidylethanolamine (PE) from E. coli, L-α-phosphatidylglycerol (PG) from E. coli, cardiolipin (CL) from E. coli, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1-palmitoyl-2-(9,10-dibromo)stearoyl-sn-glycero-3-phosphocholine (16:0–18:0(9-10Br)PC), and 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (18:1 CL). All detergents were purchased from Anatrace: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), octylglucoside (OG), polyoxyethylene(9)dodecyl ether (C12E9), n-Decyl-α-D-Maltoside (DM), n-Dodecyl-α-D-Maltoside (DDM), lauryldimethylamine-N-Oxide (LDAO). The Imperial Protein Stain solution and micro-bicinchoninic acid assay (BCA) were purchased from Thermo Fisher. The QuikChange Lightning site-directed mutagenesis kit was purchased from Agilent Technologies. 1,5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS) and 3-(N-maleimidopropionyl) biocytin (MPB) were purchased from Molecular Probes. 4-Nitrophenyl- β-D-galactopyranoside (NPG) and N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM) were purchased from Sigma Aldrich. β-D-Galactopyranosyl 1-thio-β-D-galactopyranoside (TDG) was obtained from Cayman Chemicals.
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