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7 protocols using quercetin 3 rutinoside hydrate

1

Oral Dosage for Thrombosis

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Example 3

Methylsulfonylmethane (250 mg, as dimethyl sulfone, 98%, MilliporeSigma, St. Louis, Mo.), rutin (500 mg, as quercetin-3-rutinoside hydrate, ≥94%, MilliporeSigma, St. Louis, Mo.), and docosahexanoic acid (500 mg, as docosahexanoic acid single-cell oil, DHASC®, Martek Biosciences Corp., Columbia, Md.) are combined and deposited in a gelatin-based soft capsule and sealed, thereby forming an oral dosage THROMBX™ composition.

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2

Oral THROMBX™ Composition Formulation

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Example 4

Methylsulfonylmethane (250 mg, as dimethyl sulfone, 98%, MilliporeSigma, St. Louis, Mo.), rutin (500 mg, as quercetin-3-rutinoside hydrate, ≥94%, MilliporeSigma, St. Louis, Mo.), and eicosapentaenoic acid (530 μL or 500 mg, as cis-5,8,11,14,17-eicosapentaenoic acid, ˜90%, MilliporeSigma, St. Louis, Mo.) are combined and deposited in a gelatin-based soft capsule and sealed, thereby forming an oral dosage THROMBX™ composition.

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3

Thrombx Oral Dosage Composition

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Example 4

Methylsulfonylmethane (250 mg, as dimethyl sulfone, 98%, MilliporeSigma, St. Louis, Mo.), rutin (500 mg, as quercetin-3-rutinoside hydrate, ≥94%, MilliporeSigma, St. Louis, Mo.), and eicosapentaenoic acid (530 μL or 500 mg, as cis-5,8,11,14,17-eicosapentaenoic acid, ˜90%, MilliporeSigma, St. Louis, Mo.) are combined and deposited in a gelatin-based soft capsule and sealed, thereby forming an oral dosage THROMBX™ composition.

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4

Oral Dosage THROMBX™ Composition

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Example 3

Methylsulfonylmethane (250 mg, as dimethyl sulfone, 98%, MilliporeSigma, St. Louis, Mo.), rutin (500 mg, as quercetin-3-rutinoside hydrate, ≥94%, MilliporeSigma, St. Louis, Mo.), and docosahexanoic acid (500 mg, as docosahexanoic acid single-cell oil, DHASC®, Martek Biosciences Corp., Columbia, Md.) are combined and deposited in a gelatin-based soft capsule and sealed, thereby forming an oral dosage THROMBX™ composition.

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5

Effects of Phytochemicals on Mast Cell Activation

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1 × 106 BMMCs/ml were cultured in triplicates with 10 ng/ml IL-3 and SCF. Cells were activated by pre-sensitizing with 1 µg/ml DNP-IgE (clone SPE7, Sigma) or vehicle (medium), followed by treatment with 200 ng/ml DNP-BSA (5 (link), 24 (link)). Increasing concentrations of curcumin (Sigma), bacitracin (Sigma), quercetin-3-rutinoside hydrate or rutin (Sigma), and propynoic acid carbamoyl methyl amide (PACMA)-31 (Sigma) or vehicle (DMSO) were added to various experimental groups for different time periods (including 30 min and 24 h) prior to challenge with DNP-BSA. Expression of cytokine genes was assessed by RT-PCR between 30 min to 1 h following activation. Assessment of secreted cytokines was performed 6 to 24 h later.
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6

Analytical Standards for Phenolic Compounds

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Analytical standards of 3-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, protocatechuic acid, quercetin-3-rutinoside hydrate, quercetin dihydrate, kaempferol, phenyllactic acid, caffeic acid, naringenin, (+)-catechin, (−)-epicatechin, p-coumaric acid and toluene were from Sigma-Aldrich (St. Louis, MO, USA). Dihydrocaffeic acid, p-hydroxyphenyllactic acid were from Santa Cruz Biotechnology (Santa Cruz, CA, USA), while luteolin was from Extrasynthese (Genay, France). HPLC-grade acetonitrile was from Sigma-Aldrich (St. Louis, MO, USA), while HPLC-grade water and LC-MS grade formic acid were purchased from VWR International (Milan, Italy).
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7

Characterization of Barley Polyphenols

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Chemicals were purchased from several companies: Gram mol (Zagreb, Croatia): potassium dihydrogen phosphate, potassium chloride, sodium hydrogen carbonate, and magnesium chloride; Kemika (Zagreb, Croatia): ammonium carbonate; Carlo Erba Reagents (Val de Reuil, France): sodium chloride; Fluka (Buchs, Switzerland): orto-phosphoric acid 85% HPLC-grade; and J.T. Baker (Gliwice, Poland): methanol HPLC grade. Polyphenol standards ((+)-catechin hydrate, (−)-epicatechin, chlorogenic and p-coumaric acid, quercetin dihydrate, quercetin-3-rutinoside hydrate, quercetin-3-glucoside), and barley β-D-glucan, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Procyanidin B1 and B2, phloretin and phloretin-2′-O-glucoside, quercetin-3-galactoside, quercetin-3-rhamnoside, and cyanidin-3-galactoside chloride were obtained from Extrasynthese (Genay, France).
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