1 × 10
6 BMMCs/ml were cultured in triplicates with 10 ng/ml IL-3 and SCF. Cells were activated by pre-sensitizing with 1 µg/ml
DNP-IgE (clone SPE7, Sigma) or vehicle (medium), followed by treatment with 200 ng/ml DNP-BSA (5 (
link), 24 (
link)). Increasing concentrations of
curcumin (Sigma),
bacitracin (Sigma),
quercetin-3-rutinoside hydrate or
rutin (Sigma), and propynoic acid carbamoyl methyl amide (PACMA)-31 (Sigma) or vehicle (DMSO) were added to various experimental groups for different time periods (including 30 min and 24 h) prior to challenge with DNP-BSA. Expression of cytokine genes was assessed by RT-PCR between 30 min to 1 h following activation. Assessment of secreted cytokines was performed 6 to 24 h later.
Krajewski D., Polukort S.H., Gelzinis J., Rovatti J., Kaczenski E., Galinski C., Pantos M., Shah N.N., Schneider S.S., Kennedy D.R, & Mathias C.B. (2020). Protein Disulfide Isomerases Regulate IgE-Mediated Mast Cell Responses and Their Inhibition Confers Protective Effects During Food Allergy. Frontiers in Immunology, 11, 606837.