Goat anti rabbit and goat anti mouse igg peroxidase conjugated secondary antibodies 31460 and 31430

The following antibodies were used for immunoblotting or immunohistochemical staining: β-actin (sc-47778) was from Santa Cruz Biotechnology, Inc. Sant (Santa Cruz, CA, USA). AMPKα (#2532) and phosphorylated AMPKα (Phospho-Thr172, #2535), mTOR (#2983), phosphorylated mTOR (Phospho-Ser2448, #5536), phosphorylated Raptor (Phospho-Ser792, #2083), phosphorylated p70 S6 kinase (Phospho-Thr389, #9234), phosphorylated 4E-BP1(Phospho-Thr37/46, #2855), cleaved PARP (#5626), cleaved caspase-3(#9661), ERK (#4696), phosphorylated ERK(Phospho-Thr202/Tyr204, #4370), p38 MAPK(#8690), phosphorylated p38 MAPK(Thr180/Tyr182, #4511), and Ki-67 (#9449) were from Cell Signaling Technology, Inc. (Danvers, MA, USA). ERK3(ab53277) was from Abcam (Cambridge, MA, USA). Goat anti-rabbit and goat anti-mouse IgG peroxidase-conjugated secondary antibodies (31460 and 31430) were from Thermo-Pierce (Rockford, IL, USA).

Cells after different treatments were collected for western blot analysis. The detailed procedure has been described previously.^{11 (link), 60 (link)} Primary antibodies were incubated at 4 °C overnight. The bands were visualized by chemiluminescence, imaged using a ChemiDoc XRS and analyzed using Image Lab (both from Bio-Rad). The following antibodies were used for immunoblotting: β-actin (sc-47778) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); cleaved caspase-3 (#9661) was from Cell Signaling Technology, Inc. (Danvers, MA, USA); PARP1 (13371-1-AP) was from the Wuhan Sanying Company (Wuhan, China); and goat anti-rabbit and goat anti-mouse IgG peroxidase-conjugated secondary antibodies (31460 and 31430) were from Thermo-Pierce (Rockford, IL, USA).