Sera was analyzed by Eve Technologies: Mouse Cytokine Array/Chemokine Array 13-plex Secondary Panel (MD13). Briefly, sera was isolated following decapitation in Microtainer serum separator tubes (BD Biosciences) followed by 2x sterile filtration with Ultrafree-MC sterile filters, 10 min at 10,000 rpm (Millipore Sigma). For analysis sera was prediluted 1:1 to a final volume of 100 μl in 1x PBS pH 7.4 and assayed/analyzed in duplicate.
Ultrafree mc sterile filters
Manufactured by Merck Group
Ultrafree-MC sterile filters are laboratory equipment designed for the filtration of samples. They are used to remove particulates, bacteria, and other contaminants from liquids prior to analysis or further processing.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ultrafree mc sterile filters
1
Cytokine Profiling in Mouse Sera
2
Multiplex Cytokine Analysis of Sera
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