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Phosphate buffer solution (pbs)

Manufactured by Sangon

Phosphate buffer solution is a type of aqueous buffer solution that maintains a stable pH level. It is commonly used in various laboratory applications to maintain a consistent pH environment for experiments and analyses.

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2 protocols using phosphate buffer solution (pbs)

1

Fluorescent Protein Conjugation Protocol

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3-Glycidyloxypropyltrimethoxysilane, Fluorescein isothiocyanate (FITC), and acetic acid were supplied by Aladdin Reagent Co., Ltd. Phosphate buffer solution (PBS) was purchased from Sangon Biotech Co., Ltd. Sulfo-Cyanine 5 succinimidyl ester (Sulfo-Cy5 NHS ester) was supplied by Duofluor Inc. FITC labeled bovine serum fibrinogen (Fg-FITC) was purchased from XI’AN QIYUE biology. PEG-NH2 (Mw = 2000) was purchased from JINGPI Technology. The silicone elastomer Sylgard 184 was obtained from Dow Corning (catalog no. 3097358–1004). Human serum albumin (catalog no. SRP6182), bovine serum albumin (catalog no. A2153), porcine serum albumin (catalog no. A1830), and rat serum albumin (catalog no. A6272) were purchased from Sigma-Aldrich.
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2

Synthesis of Amino Acid Derivatives for Biomaterial Development

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Nε‐benzyloxycarbonyl‐α‐tert‐butyl‐L‐lysine hydrochloride, Nε‐benzyloxycarbonyl‐α‐tert‐butyl‐D‐lysine hydrochloride, δ‐benzyl‐Ntert‐butoxycarbonyl‐L‐glutamic acid, and δ‐benzyl‐Ntert‐butoxycarbonyl‐D‐glutamic acid were supplied by Shanghai Hanhong Chemical Co., Ltd. 1‐(3‐Dimethylaminopropyl)−3‐ethylcarbodiimide hydrochloride (EDCl), 2‐hydroxybenzotriazole (HOBt), trifluoroacetic acid (TFA), anhydrous dimethylformamide (DMF), 33 wt% HBr/HOAc solution, 1H,1H,2H,2H‐Perfluorooctyl‐trichlorosilane, n‐hexadecane, fluorescein isothiocyanate (FITC), Ammonium persulfate (APS), N,N,N′,N′‐Tetramethyl ethylenediamine (TEMED), and triethylamine (TEA) were supplied by Aladdin Reagent Co., Ltd. o‐Phenylenediamine (OPD) and Fluorescamine was purchased from Macklin Reagent Co., Ltd. Phosphate buffer solution (PBS) was supplied by Sangon Biotech Co., Ltd. Trypsin (Catalog. CR25200) was purchased from Cienry Co., Ltd. Fibrinogen and 4‐Morpholineethanesulfonic acid (MES) was purchased from Sigma‐Aldrich. Poly(ethyleneglycol) diacrylate (PEGDA), Mn = 2,000 Da was obtained from JenKem. The Silhouette infusion set (Silhouette Paradigm MMT‐381) was purchased from GEMCO Medical.
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