5500 qtrap triple quadrupole ms ms system

Mass spectrometer parameters were used according to Fischer et al. [35 (link)], with minor modifications. LC-MS analysis was performed on an Agilent 1260 Infinity II HPLC-system (Agilent Technologies Deutschland GmbH, Waldbronn, Germany) coupled to a 5500 QTrap triple quadrupole MS/MS system (AB Sciex Germany GmbH, Darmstadt, Germany). Separation of ‘solution 2’ (the SQDG fraction) was achieved with a Kinetex^® C18 column (5 µm, 100 Å, 150 mm x 2.1 mm; Phenomenex Ltd., Aschaffenburg, Germany), using a constant flow rate of 0.3 mL min⁻¹. Eluent A was water and eluent B was acetonitrile/water (9/1; v/v), each with 10 mM ammonium acetate. The elution started with 3% eluent B for 2 min and linearly increased to 99% eluent B within 4 min, which was kept constant for 32 min. Then, the composition was readjusted to 3% eluent B within 2 min, followed by 4 min of re-equilibration. The SQDG derivative m/z 846 was used as internal standard in a concentration of 0.5 µg·mL⁻¹.

Mass spectrometer parameters were according to Fischer et al. [8 (link)], with slight modifications. LC-MS analytic was performed on Agilent 1260 Infinity II HPLC-system (Agilent Technologies Deutschland GmbH, Waldbronn, Germany) coupled with 5500 QTrap triple quadrupole MS/MS system (AB Sciex Germany GmbH, Darmstadt, Germany). Separation of ‘solution 2’ (SQDG) was achieved with Kinetex^® C18 column (5 μm, 100 Å, 150 mm × 2.1 mm; Phenomenex Ltd., Aschaffenburg, Germany), using a constant flow rate of 0.3 mL/min. Eluent A was water and eluent B was acetonitrile/water (9/1; v/v), each with 10 mM ammonium acetate. The elution started with 3% eluent B for 2 min and linearly increased to 99% eluent B within 4 min, which was kept constant for 32 min. Then, the composition was readjusted to 3% eluent B within 2 min, followed by 4 min of re-equilibration. The SQDG derivative 816 was used as standard in the concentration range of 0.1–1 µg mL⁻¹.