Mouse cd4 cd62l t cell isolation kit

Tobacco was purchased from Henan Tobacco Industry (Hongqi Canal^® Filter tip cigarette; tobacco type, tar: 10 mg; nicotine content: 1.0 mg; carbon monoxide: 12 mg, Zhengzhou, China). Klebsiella pneumoniae (strain ID: 46114) was obtained from the National Center for Medical Culture Collection (CMCC, Beijing, China). Aminophylline was purchased from Shandong Xinhua Pharmaceutical Co., LTD. (Shandong, China). KW6002 was obtained from MedChem Express (Shanghai, China). Anti-CD4, IL-17A, CD25, and Foxp3 antibodies were obtained from eBioscience, Inc. (Affymetrix, CA, USA). Rat IL-1β, tumor necrosis factor (TNF)-α, IL-6, IL-17A, and IL-10, ELISA kits were purchased from Boster Biological Engineering (Wuhan, China). TGF-β, IL-6, and anti-CD3/CD28 antibodies were obtained from BD Biosciences (Franklin Lakes, USA). The mouse CD4⁺ CD62L⁺ T Cell Isolation Kit was obtained from Miltenyi Biotec Inc. (Miltenyi, CA, USA). Anti-A2aR, Foxp3, RORγ, p-STAT3 (Tyr705), p-STAT5 (Tyr694), STAT3, and STAT5 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HiScript QRTSuperMix and AceQ quantitative polymerase chain reaction (qPCR) SYBR Green Master Mix were purchased from Vazyme Biotech (Nanjing, China).