Mouse neutrophil elastase elisa kit

Manufactured by Cusabio

Sourced in China

The Mouse Neutrophil Elastase ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse neutrophil elastase levels in biological samples.

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Lab products found in correlation

Enzchek myeloperoxidase activity assay kit, by Thermo Fisher Scientific (2 mentions)

3 protocols using mouse neutrophil elastase elisa kit

Neutrophil Elastase Production Assay

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Neutrophils isolated from bone marrow were cultured (1 × 10⁶ cells/well) and stimulated with fMLF (1 μmol/L), mtDNA (5 μg/mL) or mitochondria (100 μg/mL) for 2 hours in a 6‐well plate at 37°C. The elastase production in the supernatant was measured by enzyme‐linked immunosorbent assay (ELISA) using a Mouse neutrophil elastase ELISA Kit (CUSABIO Life Science, Wuhan, China).

Liu L., Mao Y., Xu B., Zhang X., Fang C., Ma Y., Men K., Qi X., Yi T., Wei Y, & Wei X. (2019). Induction of neutrophil extracellular traps during tissue injury: Involvement of STING and Toll‐like receptor 9 pathways. Cell Proliferation, 52(3), e12579.

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Neutrophil Activation and Secretion Assay

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Mouse bone marrow neutrophils were seeded in 24-well plate with RPMI complete medium at a concentration of 5 × 10⁶/ml. Then, mitochondria (200 µg/ml), mtDNA (2.5 µg/ml) and fMLF (1 µM) were added to the cells, and incubated at 37°C for 2 h. Activated neutrophils released myeloperoxidase (MPO) and elastase into medium. The medium was collected, centrifuged and the supernatants were kept in -80°C. MPO production and elastase release were determined using EnzChek Myeloperoxidase Activity Assay Kit (Invitrogen) and Mouse neutrophil elastase ELISA Kit (CUSABIO Life science, China), respectively.

Yuan X., Nie W., He Z., Yang J., Shao B., Ma X., Zhang X., Bi Z., Sun L., Liang X., Tie Y., Liu Y., Mo F., Xie D., Wei Y, & Wei X. (2020). Carbon black nanoparticles induce cell necrosis through lysosomal membrane permeabilization and cause subsequent inflammatory response. Theranostics, 10(10), 4589-4605.

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Neutrophil Activation by fMLF and mtDNA

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The isolated mouse bone marrow neutrophils were placed in a 24-well plate at a density of 5 × 10⁶ cells in 1 ml of dHBSS. fMLF (1 μM), mtDNA (5 μg/ml) were added to the cells and incubated for 4 h. MPO released into culture medium was determined using EnzChek Myeloperoxidase Activity Assay Kit (Invitrogen). Release of neutrophil elastase was measured by enzyme-linked immunosorbent assay (ELISA) using Mouse neutrophil elastase ELISA Kit (CUSABIO Life science, China).

Wei X., Shao B., He Z., Ye T., Luo M., Sang Y., Liang X., Wang W., Luo S., Yang S., Zhang S., Gong C., Gou M., Deng H., Zhao Y., Yang H., Deng S., Zhao C., Yang L., Qian Z., Li J., Sun X., Han J., Jiang C., Wu M, & Zhang Z. (2015). Cationic nanocarriers induce cell necrosis through impairment of Na+/K+-ATPase and cause subsequent inflammatory response. Cell Research, 25(2), 237-253.

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