Model 17 a

Gas chromatographic (GC) analyses were run on a Shimadzu gas chromatograph, Model 17-A equipped with a flame ionization detector (FID), and with operating software Class VP Chromatography Date System version 4.3 (Shimadzu). Analytical conditions: SPB-5 capillary column (15 m × 0.10 mm × 0.15 µm), helium as carrier gas (1 mL/min). Injection in split mode (1:200), injected volume 1 µL (4% essential oil/CH₂Cl₂v/v), injector and detector temperature 250 and 280 °C, respectively. Linear velocity in column 19 cm/sec. The oven temperature was held at 60 °C for 1 min, and then programmed from 60 to 280 °C at 10 °C/min; then 280 °C for 1 min. Each oil sample was analyzed in triplicate. Percentages of compounds were determined from their peak areas in the GC-FID profiles.
Gas–chromatography-mass spectrometry (GC–MS) was carried out in the fast mode on a Shimadzu GC–MS mod. GCMS-QP5050A, with the same column and the same operative conditions used for analytical GC–FID, operating software GCMS solution version 1.02 (Shimadzu). Ionization voltage 70 eV, electron multiplier 900 V, ion source temperature 180 °C. Mass spectra data were acquired in the scan mode in m/z range 40–400. The same oil solutions (1 µL) were injected with the split mode (1:96).

Gas chromatographic (GC) analyses were run on a Shimadzu Model 17-A gas chromatograph equipped with a flame ionization detector (FID) and with the operating software Class VP Chromatography Data System version 4.3 (Shimadzu, Kyoto, Japan). The analytical conditions included an SPB-5 capillary column (15 m × 0.10 mm × 0.15 μm) and helium as the carrier gas (1 mL/min). Injection was performed in split mode (1:200) with an injection volume of 1 μL (4% essential oil/CH2Cl2 v/v) and with injector and detector temperatures of 250 and 280 °C, respectively. The linear velocity in the column was 19 cm/s. The oven temperature was held at 60 °C for 1 min and then followed a program with an increase from 60 to 280 °C at 10 °C/min and then a hold at 280 °C for 1 min. The percentages of the compounds were determined from their peak areas in the GC-FID profiles.
Gas chromatography–mass spectrometry (GC-MS) was carried out in fast mode on a Shimadzu GC-MS (model GCMS-QP5050A) with the same column and operating conditions used for analytical GC-FID and with the operating software GCMS Solution version 1.02 (Shimadzu). The ionization voltage was 70 eV, the electron multiplier was 900 V, and the ion source temperature was 180 °C. Mass spectra were acquired in the scan mode in the m/z range 40–400. The same oil solutions (1 μL) were injected with the split mode (1:96).