Equal amounts of each cell lysate were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE), after which resolved proteins were transferred to polyvinylidenedifluoride (PVDF) membranes. The membranes were incubated with iNOS primary antibody (Santa Cruz) diluted in TBST (20 mM Tris, 134 mM NaCl, 0.02 % Tween 20). The primary antibodies were then probed with horseradish peroxidase-conjugated secondary antibodies and visualized by exposure to an enhanced chemiluminescence reagent.
Inos primary antibody
Manufactured by Santa Cruz Biotechnology
The INOS primary antibody is a research-use only reagent produced by Santa Cruz Biotechnology. It is designed to detect the inducible nitric oxide synthase (iNOS) protein. The antibody can be used in various immunological techniques to identify and study the expression of iNOS in biological samples.
Automatically generated - may contain errors
2 protocols using inos primary antibody
1
Western Blot Analysis of iNOS
2
Notch Signaling Regulation in LPS-Induced
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LPS from Escherichia coli (0111:B4), AA and DAPT were purchased from Sigma–Aldrich. Dll4 was obtained from R&D Systems. β-Actin and Hes1 primary antibody were obtained from Abcam, Notch3 and iNOS primary antibody were from Santa Cruz Biotechnology. Phospho-C-Jun N-terminal kinase (JNK) (Thr183/Tyr185) antibody, phospho-extracellular signal-regulated kinase (ERK)1/2 (Thr202/Tyr204) antibody, phospho-p38 (Thr180/Tyr182) antibody, and phospho-IκBα antibody were purchased from Cell Signaling Technology. All secondary antibodies were from Boster Biological Technology. All other reagents were from commercial suppliers and of standard biochemical quality.
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