Aacocf3

Crystallized Calloselasma rhodostoma venom, lipopolysaccharides from Escherichia coli O111:B4 (LPS), Histopaque 1077, 3,30-diaminobenzidine tablets, hydrogen peroxide 30%, ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), HEPES, Triton X-100, leupeptin, aprotinin, phenylmethylsulfonyl fluoride (PMSF), sodium orthovanadate, protease and phosphatase inhibitor cocktail, ethylenediaminetetraacetic acid disodium salt dihydrate (Na₂ EDTA), bicinchoninic acid protein assay Kit (BCA), oil Red O, AACOCF3 (arachidonyl trifluoromethyl ketone), anti-mouse Fab specific-FITC and anti-β-actin were purchased from Sigma Chem. Co. (Misssouri, USA). CAY10650, A922500, prostaglandin E₂ ELISA Kit, anti-COX-1 and anti-COX-2 were purchased from Cayman Chemical (Michigan, USA). Anti-cPLA₂-α, anti-PTGES and anti-p-cPLA₂-α were purchased from Santa Cruz Biotechnology (Texas, USA). PureLink kit, SuperScript III Reverse Transcriptase, GeneChip WT PLUS Reagent Kit and GeneChip Clariom S Array Human were purchased from Thermo Fisher Scientific (Massachusetts, USA). iTaq Universal SYBR Green Supermix were purchased from Bio-Rad (California, USA). All salts and reagents used obtained from Merck Millipore (Darmstadt, Germany) with low endotoxin or endotoxin-free grades.