Xtimate c18

Manufactured by Hill-Rom

The Xtimate C18 is a laboratory equipment product offered by Hill-Rom. The core function of the Xtimate C18 is to perform chromatographic separation and analysis of chemical compounds.

Automatically generated - may contain errors

Lab products found in correlation

Lc 20at quaternary pump, by Shimadzu (1 mentions) Sil 20a xr autosampler, by Shimadzu (1 mentions) Hplc system, by Shimadzu (1 mentions) High performance liquid chromatography system, by Shimadzu (1 mentions) G2 q tof mass spectrometer, by Waters Corporation (1 mentions) Silica gel 60 f254, by Merck Group (1 mentions) 400 mhz spectrometer, by Bruker (1 mentions) Orbitrap fusion lumos tribrid, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Welch Xtimate C18 (3 citations)

5 protocols using xtimate c18

High-Performance Liquid Chromatography of Herb Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chromatographic conditions used in the present study were as follows: The column used was the Welch Xtimate® C18 (4.6 × 250.0 mm, 3 μm), the detection wavelength was 270 nm, and the column temperature was 30 °C. In addition, the loading volume was 10 μL, and the herb underwent gradient elution with acetonitrile-30 mmol/L ammonium bicarbonate (containing 0.7% ammonia and 0.1% triethylamine) as the mobile phase, with a flow rate of 1 mL/min. The specific chromatogram is shown in Figs. 1, and 3 shows the chromatogram of the reference substances (Figs. 2, 3).
Fig. 1

High-performance liquid chromatogram. The numbered peaks represent: 2. Pharmacorhizine hydrochloride, 3. Tetrandrine hydrochloride, 4. Epiberberine hydrochloride, 5. Coptidine hydrochloride, 6. Palmatine hydrochloride, and 7. Berberine hydrochloride

Fig. 2

Contour and response surface

Fig. 3

Chromatogram of reference substances: 1. Epaperberine, 2. Coptidine, 3. Palmatine, and 4. Berberine

Wang Y., Qin W., Yang Y., Bai H., Wang J., Zhang X., Guo Y., Hua L, & Yang Y. (2022). A study on the processing technology for Rhizoma Coptidis. BMC Biotechnology, 22, 3.

+ Open protocol

+ Expand

HPLC-UV Analysis of Alkaloids in Botanical Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dried powder samples (0.02 g) were accurately weighed and extracted with hydrochloric acid-methanol solution (10 mL, 1:100, v/v) for 30 min in an ultrasonic bath at room temperature. Then, the decrease in weight was replenished with the extraction solvent. The sample solution was filtered through a 0.45 μm membrane filter prior to the HPLC-UV analysis. A Shimadzu HPLC system (Shimadzu, Kyoto, Japan) equipped with a LC-20AT quaternary pump, a SIL-20A XR autosampler, a CTO-20AC column oven and a SPD-20A UV/Vis detector was used to determine the objective compounds. The chromatographic column Xtimate C18 (250 × 4.6 mm, 5 μm, Welch, Shanghai, China) was applied to perform chromatographic separation and the column temperature was constantly kept at 25 °C The binary mobile phase consisted of acetonitrile (A) and 30 mmol/L ammonium bicarbonate solution containing 0.7% ammonia and 0.25% triethylamine (B) at a continuous flow rate of 1 mL/min in the following gradient program: 0–15 min, 10%–25% A; 15–25 min, 25%–30% A; 25–50 min, 30%–45% A. The injection volume was 5 μL and the detection wavelength was acquired at 270 nm. The contents of eight alkaloids (berberine, palmatine, coptisine, epiberberine, columbamine, jatrorrhizine, magnoflorine and groenlandicine) in each sample were calculated from standard curves.

Zhong F., Shen C., Qi L, & Ma Y. (2018). A Multi-Level Strategy Based on Metabolic and Molecular Genetic Approaches for the Characterization of Different Coptis Medicines Using HPLC-UV and RAD-seq Techniques. Molecules, 23(12), 3090.

+ Open protocol

+ Expand

Peptide Separation and Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The peptides were desalted and dissolved in high concentration organic solutions with shaking for 15 min at room temperature, and separated by centrifugation for 10 min at 16,000 g. Further high performance liquid chromatography separation details are presented in our previously published paper [32] (link). Briefly, both the supernatant and pellet peptides were separated on a self-packed capillary column N (180 μm × 4 cm, 3 μm, 120 Å) packed with Xtimate C18 (Welch, TX) at a flow rate of 8 μl/min on a Shimadzu high performance liquid chromatography system. The mobile phases consisted of A (deionized water) and B (50% acetonitrile, pH 9.0) with the following 70-min elution gradient: 0 min, 10% B; 5 min, 10% B; 60 min, 40% B; 62 min, 90% B; 64 min, 90% B; 65 min, 10% B; and 70 min, 10% B. The peptides were then collected and freeze-dried.

Chen Q., Zhang Y., Zhang K., Liu J., Pan H., Wang X., Li S., Hu D., Lin Z., Zhao Y., Hou G., Guan F., Li H., Liu S, & Ren Y. (2022). Profiling the Bisecting N-acetylglucosamine Modification in Amniotic Membrane via Mass Spectrometry. Genomics, Proteomics & Bioinformatics, 20(4), 648-656.

+ Open protocol

+ Expand

Characterization of Lapatinib Derivatives

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The general protocols employed in the synthetic procedures, structure elucidation of the new chemical structures, and purity of the newly synthesized lapatinib derivatives were performed as reported earlier, with some modifications [37 (link),38 (link),39 (link),40 (link)]. In brief, all acquired solvents and reagents were utilized without additional purification. Dimethyl sulfoxide (DMSO) was used as a solvent for NMR analysis. ¹H NMR spectra were acquired using Bruker 400 MHz spectrometer, with chemical shifts being determined in parts per million (ppm) and coupling constants in Hz. ¹³C NMR spectra were acquired using Varian 100 MHz spectrometer (Varian Medical Systems, Inc., Palo Alto, CA, USA). The G2 QTOF mass spectrometer (Waters Corporation, Milford, MA, USA) was employed to produce the mass spectra, high-resolution mass spectrometry (HRMS, ESI-MS). Reaction monitoring was performed using TLC on 0.25 mm silica plates (E. Merck; silica gel 60 F₂₅₄). To verify the purity of the target compounds, high-performance liquid chromatography (HPLC); System: Waters Corp. 2695 with PAD 996, λ = 281 nm; Column: Welch Xtimate C₁₈ 150 mm × 4.6 mm × 5 μm; Condition: mobile phase A: 0.05% TFA in water, mobile phase B: CH₃CN; Gradient: 90% of Mobile phase B in 30 min. The melting points (M.p.) were assessed with Thermo Scientific 9200 (Rise 10 °C per minute).

Elkamhawy A., Son S., Lee H.Y., El-Maghrabey M.H., Hamd M.A., Alshammari S.O., Abdelhameed A.A., Alshammari Q.A., Abdeen A., Ibrahim S.F., Mahdi W.A., Alshehri S., Alnajjar R., Choi W.J., Al-Karmalawy A.A, & Lee K. (2022). Design, Synthesis, Biological Evaluation, and Molecular Dynamics Studies of Novel Lapatinib Derivatives. Pharmaceuticals, 16(1), 43.

+ Open protocol

+ Expand

Analytical Characterization of Agrobacterium sp. DW-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ultraviolet spectrum scanning was performed on the Agilent Cary60 spectrophotometer. 3-Hydroxypyridine and the degradation intermediates were determined by HPLC with diode array detection, using reverse-phase column (Welch Xtimate C18, 4.6 mm × 250 mm, 5 μm) at 30 °C. The mobile phase was 20% (v/v) methanol and 80% distilled water at a flow rate of 1.0 mL/min. Mass spectrometry (MS) analysis was performed on an Orbitrap Fusion Lumos Tribrid (Thermo Fisher) equipped with electrospray ionization (ESI) sources, using reverse-phase column (Agilent ZORBAX RRHD Eclipse Plus 95Å C18 2.1 × 100 mm, 1.8 µm). The mobile phase contained 80% (v/v) 0.05% (w/v) formic acid and 20% (v/v) methanol at a flow rate of 0.2 mL/min. Both positive and negative electrospray ionization analysis with the continuous full scanning from m/z 50 to 500 were collected. Samples for HPLC and LC–MS analysis were treated by adding 9 volume of methanol and centrifuged at 10,000×g for 5 min, and then the supernatant was filtered by a 0.22 μm filter.
Nucleotide sequence accession numbers: The 16S ribosomal RNA gene sequence of Agrobacterium sp. DW-1 is available in GenBank under Accession Number MK402166.

Zhao S., Hu C., Guo L., Li K, & Yu H. (2019). Isolation of a 3-hydroxypyridine degrading bacterium, Agrobacterium sp. DW-1, and its proposed degradation pathway. AMB Express, 9, 65.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!