Derivatized samples were analyzed with an Agilent 7890B GC system coupled to a 7000 Triple Quadrupole GC-MS system. The column was Phenomenex ZB-1701 column (30 m x 0.25 mm x 0.25 μm), with the following oven program: initial temperature 50°C/ hold 2 min; increment at 10°C/min to 140°C/ hold 0 min; increment at 20°C/min to 182°C/ hold 1 min; and increment at 50°C/min to 280°C/ hold 0 min. Run time 16.1 min. Samples (1 μL) were injected using split mode (20:1, 28 mL/min split flow). The column gas flow was held at 1.4 mL/min of He. The temperature of the inlet was 280°C, the interface temperature 230°C, and the quadrupole temperature 150°C. The column was equilibrated for 2 min before each analysis. The mass spectrometer was operated in single-ion monitoring (SIM) mode between 9.9 and 14.0 min. A segment for each compound was defined: benzyl-formate at 9.9 min, fragments m/z 136 and 137; benzyl-acetate at 11.0 min, fragments m/z 150, 151, 152 and 153; benzyl-propionate at 12.2, fragments m/z 164, 165, 166 and 167; and benzyl-butyrate at 13 min, fragments m/z 178,179,180, and 182. All segments at 2.5 cycles/s. Agilent MassHunter Workstation Software (version B.07.01 SP1) was employed for automated data processing, using peak area for absolute concentrations calculations.
7000 triple quadrupole gc ms system
Manufactured by Agilent Technologies
Sourced in United States
The 7000 Triple Quadrupole GC-MS system is a highly sensitive and selective mass spectrometry instrument designed for gas chromatography. It combines a gas chromatograph and a triple quadrupole mass spectrometer to provide accurate quantitative and qualitative analysis of complex samples.
Automatically generated - may contain errors
Lab products found in correlation
7890b gc system, by Agilent Technologies (2 mentions)
Zb 1701 column, by Phenomenex (2 mentions)
Db 17ms capillary column, by Agilent Technologies (2 mentions)
Db 5ms, by Agilent Technologies (2 mentions)
Db 1ms, by Agilent Technologies (2 mentions)
Agilent 7890b gc system, by Agilent Technologies (2 mentions)
Masshunter workstation software, by Agilent Technologies (1 mentions)
Masshunter b 06, by Agilent Technologies (1 mentions)
6 protocols using 7000 triple quadrupole gc ms system
1
GC-MS Analysis of Derivatized Compounds
2
GC-MS Quantification of Acetate Isotopomers
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The acetate samples were analyzed with an Agilent 7890B GC system coupled to a 7000 Triple Quadrupole GC-MS system. The column was Phenomenex ZB-1701 column (30 m × 0.25 mm × 0.25 μm), with an oven program as described in Table 1 . Samples (2 μL) were injected using split mode (0.5 bar, 25 mL/min split flow). The column gas flow was held at 1.0 mL of He per min. The temperature of the inlet was 280 °C, the interface temperature 230 °C, and the quadrupole temperature 200 °C. The column was equilibrated for 2 min before each analysis. The mass spectrometer was operated in scan mode between 2.2 and 2.7 min with a mass range of 30–150 AMU at 1.47 scans/s. Agilent Mass Hunter B.06.00 software together with R-based MetabQ software were employed for automated data processing using peak heights of m/z 61, 63, and 64 ions used to quantify 12C, U-13C, and 2H3-acetate, respectively (the peak shapes were consistently highly symmetric, and using either peak area or peak heights gave equivalent results) [30 (link)]. Peak heights of 12C and U-13C acetate were compared to the 2H3-acetate peak height, and absolute concentrations were obtained from a calibration curve.
GC temperature program for acetate analysis
| Start temperature (°C) | Ramp (°C/min) | End temperature (°C) | Hold time (min) |
|---|---|---|---|
| 45 | – | 45 | 0.8 |
| 45 | 25 | 60 | 0 |
| 60 | 50 | 190 | 0 |
3
GC-MS Protocol for Compound Analysis
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GC–MS analysis was performed on a 7000 triple quadrupole GC–MS system (Agilent, Santa Clara, CA, USA) equipped with a DB-1ms, DB-5ms or DB-17ms capillary column (30 m × 0.25 mm I.D., film thickness 0.25 μm; Agilent J&W, Folsom, CA, USA). The oven temperature was maintained at 80 °C for 1 min following the injection of each sample and then increased to 300 °C at a rate of 15 °C/min. The injection port and interface temperature were set at 250 °C. Helium was used as the carrier gas at a flow rate of 1.0 ml/min. One microliter of sample solution was injected in splitless mode.
For EI mode, the ionization energy was 70 eV, the ion source temperature was 230 °C, and the scan mass range was m/z 40–400.
For CI mode, the ionization energy was 70 eV, the ion source temperature was 250 °C, and the scan mass range was m/z 43–400. Methane was used as a reactant gas.
For EI mode, the ionization energy was 70 eV, the ion source temperature was 230 °C, and the scan mass range was m/z 40–400.
For CI mode, the ionization energy was 70 eV, the ion source temperature was 250 °C, and the scan mass range was m/z 43–400. Methane was used as a reactant gas.
4
Acetate Derivatization for GC-MS Analysis
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For acetate derivatization, 200 μl cell culture medium was collected into a 2 mL microfuge tube, followed by 40 μl 2H3-acetate (1 mM), 1-propanol (50 μl), and pyridine (50 μl). Tube was put on ice for 5 min and 100 μl NaOH (1 M), 30 μl methyl-chloroformate were added. After overtaxing for 20 s, add 300 μl of tert-butyl methyl ether (MTBE) to the tube and vortex again. The samples were then centrifuged at 10,000 × g for 5 min. The upper layer was transferred to GC-MS vials and analyzed with an Agilent 7890B GC system and 7000 Triple Quadrupole GC-MS system. Data were collected and analyzed as previously described22 (link).
5
GC-MS Analysis of Complex Samples
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GC–MS was performed on a 7000 Triple quadrupole GC–MS system (Agilent, Santa Clara, CA, USA) equipped with a DB-1ms, a DB-5ms, or a DB-17ms capillary column (30 m × 0.25 mm I.D., film thickness 0.25 μm, Agilent J&W, Folsom, CA, USA). The oven temperature was maintained at 80 °C for 1 min following injection and then raised to 300 °C at a rate of 15 °C/min. The injection port and interface temperature were set at 250 °C. Helium was used as the carrier gas at a flow rate of 1.0 ml/min. One microliter of sample solution was injected in splitless mode.
For EI mode, the ionization energy was 70 eV, the ion source temperature was 230 °C, and the scan mass range was m/z 40–400. For CI mode, the ionization energy was 70 eV, the ion source temperature was 250 °C, and the scan mass range was m/z 43–400.
For EI mode, the ionization energy was 70 eV, the ion source temperature was 230 °C, and the scan mass range was m/z 40–400. For CI mode, the ionization energy was 70 eV, the ion source temperature was 250 °C, and the scan mass range was m/z 43–400.
6
Acetate Derivatization for GC-MS Analysis
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For acetate derivatization, 200 μl cell culture medium was collected into a 2 mL microfuge tube, followed by 40 μl 2H3-acetate (1 mM), 1-propanol (50 μl), and pyridine (50 μl). Tube was put on ice for 5 min and 100 μl NaOH (1 M), 30 μl methyl-chloroformate were added. After overtaxing for 20 s, add 300 μl of tert-butyl methyl ether (MTBE) to the tube and vortex again. The samples were then centrifuged at 10,000 × g for 5 min. The upper layer was transferred to GC-MS vials and analyzed with an Agilent 7890B GC system and 7000 Triple Quadrupole GC-MS system. Data were collected and analyzed as previously described22 (link).
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