5-Bromo-2′-Deoxyuridine (BrdU), Evans blue dye, tamoxifen, rabbit anti-laminin antibody, and 2,3,5-triphenyl-tetrazolium chloride (TTC) were from Sigma-Aldrich (St. Louis, MO). Osmium Tetroxide and Uranyl Acetate were from Electron Microscopy Sciences (Hatfield, PA). Propylene oxide, Polybed 812 epoxy resin were from Polysciences (Warrington, PA). Toluidine Blue and Potassium Ferricyanide was from Fischer chemicals. Rabbit anti-Aquaporin 4 (AQP4) antibody was from Milipore (Billerica, MA). Mouse anti-GFAP antibodies were from Cell Signaling Technology (Danvers, MA). Sheep anti-BrdU antibody and mouse monoclonal antibody to S100β were from Abcam (Cambridge, MA). Anti-MMP-9 and anti NHE1 andibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse monoclonal antibodies to Occludin, and Claudin antibody were from Thermoscientific Life Technologies Corporation (Grand Island, NY). Donkey anti-goat Alexa Fluor® 488-conjugated IgG, goat anti-rat Alexa Fluor® 488-conjugated IgG, donkey anti-rabbit Alexa Fluor® 546-cojugated IgG, goat anti-rabbit Alexa Fluor® 546-cojugated IgG, and TO-PRO®-3 iodide were from Invitrogen (Carlsbad, CA).
Rabbit anti aquaporin 4 aqp4 antibody
Manufactured by Merck Group
Rabbit anti-Aquaporin 4 (AQP4) antibody is a laboratory reagent used in research applications. It is a polyclonal antibody raised in rabbits that specifically binds to the Aquaporin 4 protein, which is a water channel protein found in various tissues.
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Lab products found in correlation
Propylene oxide, by Polysciences (1 mentions)
Osmium tetroxide, by Electron Microscopy Sciences (1 mentions)
2 3 5 triphenyltetrazolium chloride, by Merck Group (1 mentions)
Polybed 812 epoxy resin, by Polysciences (1 mentions)
Mouse anti gfap antibody, by Cell Signaling Technology (1 mentions)
Sheep anti brdu antibody, by Abcam (1 mentions)
Alexa fluor 488 conjugated goat anti rat igg, by Thermo Fisher Scientific (1 mentions)
Donkey anti rabbit alexa fluor 546 cojugated igg, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit alexa fluor 546 cojugated igg, by Thermo Fisher Scientific (1 mentions)
Rabbit anti laminin antibody, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Uranyl acetate, by Electron Microscopy Sciences (1 mentions)
Mowiol, by Merck Group (1 mentions)
Rat anti ctip2 antibody, by Abcam (1 mentions)
Rabbit anti neun antibody, by Cell Signaling Technology (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions)
2 protocols using rabbit anti aquaporin 4 aqp4 antibody
1
Multimodal Imaging of Brain Tissue
2
Immunohistochemistry Protocol for Cellular Markers
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Immunohistochemistry was performed as described previously with slight modifications38 (link),39 (link). Briefly, free-floating sections were permeabilized with 0.3% Triton X-100/PBS, incubated overnight with primary antibodies in 2% bovine serum albumin (BSA)/PBS, and then washed with PBS 3 times for 10 min each. The sections were incubated with secondary antibodies and Hoechst 33342 in 2% BSA/PBS for 2 h, and then washed with PBS 3 times for 10 min each. The sections were mounted on slides with Mowiol (Sigma-Aldrich). Antibodies used were as follows: rabbit anti-aquaporin-4 (AQP4) antibody (Millipore), rabbit anti-glutamine synthetase (GS) antibody (Sigma-Aldrich), mouse anti-GS antibody (Roche), rabbit anti-NeuN antibody (Cell Signaling Technology), mouse CC1 antibody (Calbiochem), rabbit anti-phospho-Smad1/5/8 antibody (Cell Signaling Technology), rabbit anti-Cux1 antibody (Santa Cruz), rat anti-Ctip2 antibody (Abcam), donkey secondary antibodies conjugated with Cy3 (Jackson ImmunoResearch), donkey secondary antibodies conjugated with Alexa Fluor 647 (Molecular Probes) and donkey secondary antibodies conjugated with Alexa Fluor 488 (Molecular Probes).
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