Tumor removal surgery was performed 12 days (4T1 model) or 2 weeks (MDA-MB-231 model) after tumor implantation. Mice were anesthetized and transferred to a heating pad. After hair removal, a small incision was made, and the tumor was carefully detached from adjacent skin, adjacent loose tissue and visible lymph nodes were also removed. The wound was closed by using 9-mm autoclips (Braintree Scientific, Inc.). Betadine was applied to the wound, and ophthalmic ointment was used to prevent eye desiccation.
9mm autoclips
Manufactured by Braintree Scientific
The 9mm AutoClips is a laboratory equipment product used for secure fastening and sealing of samples in various scientific applications. It provides a reliable and consistent clamping mechanism to hold materials in place during experiments or storage. The core function of the 9mm AutoClips is to facilitate secure sample containment.
Automatically generated - may contain errors
7 protocols using 9mm autoclips
1
Tumor Resection in Mouse Models
2
Orthotopic Tumor Xenograft Model
3
Tumor Relapse Monitoring by Collagen Sponge
4
Tumor Relapse Monitoring by Collagen Sponge
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
500,000 cells (T-HEp3 CDK2 sensor and H2B-RFP) were resuspended in 100μl of 1X sterile DPBS and injected in the interscapular region of the neck of nude mice. 5 mice were used per group. Tumor sizes were monitored every 4 days for 19 days. At day 19 after tumor cell injections, tumors were removed following the surgery procedure described in the previous section. One group of mice received a dental sponge rehydrated in DPBS and the other group received a dental sponge rehydrated in DPBS and incubated with type III collagen 1mg/ml polymerized for 4hrs at 37°C. Dental sponge reference Gelfoam® size 4 was purchased from Pfizer. They were cut in three pieces, each animal received one piece of sponge. The wound was closed using 9mm AutoClips® (reference 205016) from Braintree Scientific. Clips were removed 10 days after surgery. Local tumor relapse was monitored up to 40 days (20 days after surgery). Mice were sacrificed with CO2 when tumors reached 1cm in diameter. At day 40, all remaining mice with no local relapses were sacrificed. For each animal, the area of resection was reopened to image residual cancer cells where the sponge was places by using a multiphoton microscope.
5
Orthotopic Tumor Xenograft Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
500,000 cells (T-HEp3 GFP or expressing CDK2 sensor and H2B-RFP) were injected in 100μl of 1X sterile DPBS orthotopically in the interscapular region of the neck of nude mice. 5 mice were used per group. Tumor sizes were monitored every 4 days for 2 weeks. At day 14 after tumor cell injections, tumors were removed under procedure sterile hood. Mice were anesthetized with isoflurane (Baxter reference # NDC 10019–360-40, provided by the Mount Sinai animal facility) and the skin around the tumor was cleaned with ethanol. Tumor was lifted from its center and an incision was made using small scissors. All macroscopic tumor tissue was removed. The wound was closed using 9mm AutoClips® (reference 205016) from Braintree Scientific. Clips were removed 10 days after surgery. Two to four weeks after surgery, mice were sacrificed with CO2 and the area of resection was reopened to image residual cancer cells (far from scar tissue) and lungs were collected and imaged to find spontaneous DTCs using a multiphoton microscope. Tumors and lungs were fixed and paraffin-embedded for tissue staining.
6
Retrograde Pancreatic Duct Injection in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized with ketamine (100 mg/kg BW) and xylazine (10 mg/kg BW) and received a subcutaneous injection of buprenorphine (0.05 mg/kg BW). A midline incision of 2-to-3 cm was made along the abdomen. The duodenum was exposed and the common bile duct was clamped caudal to the liver. The intraductal injection was performed using a 28G1/2 needle. The syringe was entered into the pancreatic duct from the duodenum through the ampulla of Vater and carefully advanced in a retrograde manner. The needle was secured by a micro clamp and 100 μl of vehicle or capsaicin (50 μg/100 μl) were slowly administered over one minute. After clamp removal, the wound on the ampulla was sealed with 3M Vetbond Tissue Adhesive (3M Animal Care). Abdominal muscle layer was closed with simple interrupted stitch with Polyamide Monofilament (B. Braun) and the overlying skin was sutured using 9 mm autoclips (Braintree Scientific).
7
Surgical Exposure of Dorsal Root Ganglia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized with ketamine (100 mg/kg BW) and xylazine (10 mg/kg BW) and received a subcutaneous injection of buprenorphine (0.05 mg/kg BW). The skin and muscles were separated exposing unilaterally the region of 11th, 12th and 13th thoracic vertebrae and the dorsal region transverse processes. To expose the DRGs, transverse processes were trimmed with a micro drill (Roboz Surgical Instrument). Whole DRGs were carefully cut with tipped Dumont #5 forceps and Vannas spring scissors (Fine Science Tools) without disrupting the spinal cord. Dorsal muscle layer was then closed with interrupted suture technique with Polyamide Monofilament (B. Braun). The overlying skin was sutured using 9 mm autoclips (Braintree Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!