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Pki 6 22

Manufactured by Santa Cruz Biotechnology
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The PKI 6-22 is a laboratory instrument designed for general protein kinase assay applications. It measures kinase activity by detecting phosphorylation of peptide substrates using a luminescent detection method. The core function of the PKI 6-22 is to provide a quantitative assessment of protein kinase activity in a high-throughput format.

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Lab products found in correlation

Staurosporine, by Bio-Techne (2 mentions) Baclofen, by Santa Cruz Biotechnology (2 mentions) Carbachol, by Bio-Techne (2 mentions) Saclofen, by Bio-Techne (2 mentions) Skf81297, by Bio-Techne (2 mentions) Nps 2143, by Bio-Techne (2 mentions) Pki 19 36, by Bio-Techne (2 mentions) Jnj 16259685, by Santa Cruz Biotechnology (2 mentions) Cinacalcet, by LGC (2 mentions) Pertussis toxin, by Merck Group (2 mentions) Celltiter blue assay, by Promega (1 mentions)

3 protocols using pki 6 22

1

Assessing PKA Inhibitor Impact on Ca46 Cells

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The BL cell line Ca46 was seeded at a density of 10.000 cells/well in duplicate. For PKA inhibition cells were treated with various concentration of the protein kinase A inhibitor PKI-6-22 (Santa Cruz Biotechnology, Texas, USA) ranging from 25nM to 500 nM. After 72h of treatment the cell viability was measured using the Cell Titer Blue Assay (Promega, Mannheim, Germany).
Kretzmer H., Bernhart S.H., Wang W., Haake A., Weniger M.A., Bergmann A.K., Betts M.J., Carrillo-de-Santa-Pau E., Doose G., Gutwein J., Richter J., Hovestadt V., Huang B., Rico D., Jühling F., Kolarova J., Lu Q., Otto C., Wagener R., Arnolds J., Burkhardt B., Claviez A., Drexler H.G., Eberth S., Eils R., Flicek P., Haas S., Humme M., Karsch D., Kerstens H.H., Klapper W., Kreuz M., Lawerenz C., Lenzek D., Loeffler M., López C., MacLeod R.A., Martens J.H., Kulis M., Martín-Subero J.I., Möller P., Nage I., Picelli S., Vater I., Rohde M., Rosenstiel P., Rosolowski M., Russell R.B., Schilhabel M., Schlesner M., Stadler P.F., Szczepanowski M., Trümper L., Stunnenberg H.G., Küppers R., Ammerpohl O., Lichter P., Siebert R., Hoffmann S, & Radlwimmer B. (2015). DNA-methylome analysis in Burkitt and follicular lymphomas identifies differentially methylated regions linked to somatic mutation and transcriptional control. Nature genetics, 47(11), 1316-1325.
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2

Electrophysiological Recordings with Diverse Pharmacological Agents

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Solutions were gravity-fed through a glass capillary (1.2 mm outer diameter) placed ~1 mm from the patch pipette tip. Most reagents were obtained from Sigma-Aldrich (Darmstadt, Germany). NPS 2143, PKI 19–36, staurosporine, saclofen, SKF 81297, carbachol, and CHPG were supplied by Tocris (Bristol, United Kingdom). PKI 6–22, JNJ 16259685, and baclofen were supplied by Santa Cruz Biotechnology (Dallas, United States). Pertussis toxin was supplied by Millipore Sigma (Burlington, Massachusetts). Cinacalcet was supplied by Toronto Research Chemicals (Toronto, Canada) and TTX by Alomone (Jerusalem, Israel). Phenytoin, carbamazepine, and CHPG were dissolved in DMSO (final concentration 0.125%). NPS 2143, calhex, MPEP, DMeOB, staurosporine, and chelerythrine chloride were dissolved in DMSO (final concentration ≤ 0.03%). JNJ 16259685 was dissolved in ethanol (final concentration 0.05%). Appropriate vehicle controls were performed for all experiments.
Mattheisen G.B., Tsintsadze T, & Smith S.M. (2018). Strong G-Protein-Mediated Inhibition of Sodium Channels. Cell reports, 23(9), 2770-2781.
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3

Electrophysiological Recordings with Diverse Pharmacological Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Solutions were gravity-fed through a glass capillary (1.2 mm outer diameter) placed ~1 mm from the patch pipette tip. Most reagents were obtained from Sigma-Aldrich (Darmstadt, Germany). NPS 2143, PKI 19–36, staurosporine, saclofen, SKF 81297, carbachol, and CHPG were supplied by Tocris (Bristol, United Kingdom). PKI 6–22, JNJ 16259685, and baclofen were supplied by Santa Cruz Biotechnology (Dallas, United States). Pertussis toxin was supplied by Millipore Sigma (Burlington, Massachusetts). Cinacalcet was supplied by Toronto Research Chemicals (Toronto, Canada) and TTX by Alomone (Jerusalem, Israel). Phenytoin, carbamazepine, and CHPG were dissolved in DMSO (final concentration 0.125%). NPS 2143, calhex, MPEP, DMeOB, staurosporine, and chelerythrine chloride were dissolved in DMSO (final concentration ≤ 0.03%). JNJ 16259685 was dissolved in ethanol (final concentration 0.05%). Appropriate vehicle controls were performed for all experiments.
Mattheisen G.B., Tsintsadze T, & Smith S.M. (2018). Strong G-Protein-Mediated Inhibition of Sodium Channels. Cell reports, 23(9), 2770-2781.
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