P gsk3a b

Manufactured by Cell Signaling Technology

P-GSK3a/b is a primary antibody that recognizes the phosphorylated forms of glycogen synthase kinase-3 alpha and beta (GSK3a and GSK3b) proteins. GSK3a and GSK3b are serine/threonine protein kinases that play important roles in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Vinculin, by Merck Group (1 mentions) P p70s6k, by Cell Signaling Technology (1 mentions) Anti au1 antibody, by Biosynth (1 mentions) P mdm2, by Cell Signaling Technology (1 mentions) Mlc2v, by Synaptic Systems (1 mentions) P 308 akt, by Cell Signaling Technology (1 mentions) P473akt, by Cell Signaling Technology (1 mentions) T akt, by Cell Signaling Technology (1 mentions) T p70s6k, by Cell Signaling Technology (1 mentions) Gapdh, by Biosynth (1 mentions) β catenin, by Merck Group (1 mentions)

2 protocols using p gsk3a b

Adenylyl Cyclase Protein Expression Analysis

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Expression of adenylyl cyclase related proteins were analyzed using
Agilent Gene Expression Microarray technique by PhalanxBio Inc. (CA, San Diego).
C1C2 protein was detected by anti-AU1 antibody (Fitzgerald, 1:2,000) or
anti-AC5/6 antibody (Santa Cruz, 1:200). Additional antibodies included: GAPDH
(Fitzgerald, 1:20,000); cMLCK (Abgent, 1:1,000); MLC2v (Synaptic Systems,
1:1,000) PKA catalytic subunit (BD Transduction, 1:1,000); p-ERK1/2, p38,
p-PKARII α and β (Santa Cruz Biotech, 1:200); PLB (Affinity
Bioreagents, 1:5,000); phospho-16-PLB (Badrilla, 1:3,000); S100A1 (Epiyomics,
1:1,000); SERCA2a antibody (Enzo, 1:1,000); phospho-αB-crystallin
(CryAB) and total CryAB antibodies (Enzo, 1:1,000); P-308-Akt, P-473-Akt, T-Akt,
p-GSK3a/b, p-MDM2, P-p70S6K, T-p70S6K, and phospho-S22/23 troponin I (TnI) (Cell
Signaling, 1:1,000 each); Vinculin (Sigma, 1:100,1000).

Gao M.H., Lai N.C., Giamouridis D., Kim Y.C., Tan Z., Guo T., Dillmann W.H., Suarez J, & Hammond H.K. (2016). Cardiac-Directed Expression of Adenylyl Cyclase Catalytic Domain Reverses Cardiac Dysfunction Caused by Sustained Beta-Adrenergic Receptor Stimulation. JACC: Basic to Translational Science, 1(7), 617-629.

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Western Blot Analysis of β-Catenin and p-GSK3

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Western blot analysis was performed as described previously using antibodies against b-catenin (Sigma-Aldrich) and p-GSK3ab (Cell Signaling Technology) and a-tubulin (EMD Millipore). Antibodies were used at a 1:1000 ratio on membranes blocked with BSA. Membranes were visualized using ImageQuant LAS 4000.

Lee Y.S., Kim T.Y., Kim Y., Lee S.H., Kim S., Kang S.W., Yang J.Y., Baek I.J., Sung Y.H., Park Y.Y., Hwang S.W., O E., Kim K.S., Liu S., Kamada N., Gao N, & Kweon M.N. (2018). Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development. Cell host & microbe, 24(6).

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