1 aminobenzotriazole

All chemical solvents, salts, and buffers were purchased from Thermo Scientific (Waltham, MA, USA). CYP isozyme inhibitors α-naphthoflavone, (+)-N-3-benzylnirvanol, ketoconazole, montelukast sodium, quinidine, sulfaphenazole, ticlopidine hydrochloride, and tranylcypromine sulfate were purchased from Millipore-Sigma. The substrate (5OH-APINACA) and CYP isozyme inhibitors (1-aminobenzotriazole and 4-methylpyrazole hydrochloride) were purchased from Cayman Chemical (Ann Arbor, MI, USA). NADPH-regenerating system components NADP disodium salt, glucose-6-phosphate dehydrogenase, and glucose-6-phosphate were purchased from Millipore-Sigma (St. Louis, MO, USA), while magnesium chloride salt was purchased from Thermo Fisher Scientific. The substrate 5F-APINACA was provided by Dr. William Fantegrossi. Human liver microsomes pooled from 150 individuals, lot#38296, and P450 contribution of 0.380 nmol/mg, were purchased from Corning (Corning, NY, USA). CD-1 male mouse liver microsomes pooled from 6424 mice, lot#2010017, and P450 contribution of 0.671 nmol/mg, were purchased from Sekisui Xenotech (Kansas City, KS, USA). Internal standard acenocoumarol was purchased from Toronto Research Chemicals (Toronto, ON, Canada). ACD/ChemSketch 2017.2.1 software (Toronto, ON, Canada) was used for rendering structures of molecules.

ATP and H₂O₂ was purchased from Wako (Tokyo, Japan). LPS was purchased from Invivogen (San Diego, CA, USA). Aspirin was purchased from Tokyo Kasei (Tokyo, Japan). 1-Aminobenzotriazole, HET0016, Zileuton, PD146176, Celecoxib, MS-PPOH, ML355, and Triacsin C were purchased from Cayman (Ann Arbor, MI, USA).