8 anilino 1 naphthalenesulfonic acid ammonium salt ans

Manufactured by Merck Group

Sourced in United States

8-anilino-1-naphthalenesulfonic acid ammonium salt (ANS) is a fluorescent dye used in biochemical and biophysical applications. It is a water-soluble compound that exhibits a shift in its fluorescence emission spectrum upon binding to hydrophobic regions of proteins or other biomolecules.

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5 protocols using 8 anilino 1 naphthalenesulfonic acid ammonium salt ans

Insulin-Protamine Binding Characterization

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Insulin human recombinant, expressed
in yeast (≥98% purity,
MW 5.8 kDa), protamine sulfate salt from salmon (MPRRRRSSSRPVRRRRRPRVSRRRRRRGGRRRR)
(MW 5.1 kDa), 8-anilino-1-naphthalenesulfonic acid ammonium salt (ANS),
and zinc sulfate monohydrate (ZnSO₄) were purchased from
Sigma-Aldrich. Na₂HPO₄, NaH₂PO₄, NaCl, and phenol were purchased from Merck Chemicals Ltd.
All experiments were performed in phosphate buffer at pH 8.0 containing
10 mM Na₂HPO₄, 10 mM NaH₂PO₄, and NaCl. The concentration of insulin was measured using an absorbance
of 276 nm on the Cary 100 UV–vis spectrophotometer. The molar
extinction coefficient used for insulin was 6200 M^–1cm^–1.^{13 (link)}

Aggarwal S., Tanwar N., Singh A, & Munde M. (2022). Formation of Protamine and Zn–Insulin Assembly: Exploring Biophysical Consequences. ACS Omega, 7(45), 41044-41057.

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Antioxidant Activity Evaluation of Polydextrose

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α-LA (L6010, purity ≥ 85%, biological source: bovine milk), 8-anilino-1-naphtha-lenesulfonic acid ammonium salt (ANS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,4,6-tris(2-pyridyl)-striazide (TPTZ) were provided by Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). PD (purity ≥ 90%, 80–120 mush, average degree: 13 (range from 2 to 120), average molecular weight: 3200 (162–5000 molecular weight: 88.7%), provided by manufacturer) was purchased from Shandong Bailong Chuangyuan Biotechnology Co., Ltd., (Dezhou, China). Sodium tetraborate decahydrate, ophthal-aldehyde (OPA) and sodium dodecyl sulfate (SDS) were all obtained from Aladdin Reagent (Shanghai, China). β-Mercapto-ethanol was acquired from Kulaibo Technology Co., Ltd., (Beijing, China). All additional chemicals were of an analytical grade, provided by Yongda Chemical Reagent Co., Ltd., (Tianjin, China).

Dai K., Wang J., Luo Y., Tu Y., Ren F, & Zhang H. (2023). Characteristics and Functional Properties of Maillard Reaction Products from α-Lactalbumin and Polydextrose. Foods, 12(15), 2866.

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Protein Characterization via Chemical Modifications

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OVA (purity ≥ 98%) was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Succinic anhydride, 2,4,6–trinitrophene sulphonic acid (TNBS), glycine, sodium dodecyl sulfates (SDS), Coomassie Brilliant Blue R–250, 5,5–dithiobis (2–nitrobenzoic acid) (DTNB), 8–anilino–1–naphthalenesulfonic acid ammonium salt (ANS), ethylenediaminetetraacetic acid (EDTA), and potassium bromide (KBr) were acquired from Sigma–Aldrich Co. LLC (Shanghai, China). Albumin bovine (BSA, purity ≥98%) was obtained from BioFroxx (Einhausen, Germany). All other reagents used in this study were of analytical grade.

Hu G., Zhang J., Wang Q., Ma M., Ma L, & Li S. (2022). Succinylation Modified Ovalbumin: Structural, Interfacial, and Functional Properties. Foods, 11(18), 2724.

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Lipid Bilayer Composition Analysis

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FL, lignoceric acid, palmitic acid, boric acid, potassium chloride, sodium hydroxide, chloroform, m ethanol, and ethanol were purchased from Wako Pure Chemicals Industries, Ltd. (Osaka, Japan). Cholesterol, cholesteryl sulfate, octacosanoic acid, 1,6-diphenyl-1,3,5-hexatriene (DPH), N,N,N-trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenyl-ammonium p-toluenesulfonate (TMA-DPH), and 8-anilino-1-naphthalenesulfonic acid ammonium salt (ANS) were from Sigma-Aldrich (St. Louis, MO, USA). Ceramide type III and ceramide type VI were from Evonik Industries AG (Essen, Germany). These reagents were used without further purification.

Sakdiset P., Kitao Y., Todo H, & Sugibayashi K. (2017). High-Throughput Screening of Potential Skin Penetration-Enhancers Using Stratum Corneum Lipid Liposomes: Preliminary Evaluation for Different Concentrations of Ethanol. Journal of Pharmaceutics, 2017, 7409420.

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Holo α-Lactalbumin Purification Protocol

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Commercially lyophilized preparation of holo α-Lactalbumin (from bovine milk) was purchased from Sigma Chemical Co (St Louis, MO, USA). Ficoll 70, trichloroacetic acid (TCA), Thioflavin T (ThT), 8-Anilino-1-naphthalenesulfonic acid ammonium salt (ANS), Ethylenediaminetetraacetic acid (EDTA) and sodium salt of cacodylic acid were also obtained from Sigma Chemical Co. Potassium chloride (KCl) and sodium acetate were obtained from Merck. Calcium chloride was obtained from G-Biosciences. These and other chemicals, which were of analytical grade, were used without further purification.

Mittal S, & Singh L.R. (2014). Macromolecular Crowding Induces Holo α-Lactalbumin Aggregation by Converting to Its Apo Form. PLoS ONE, 9(12), e114029.

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