Log In Sign up
The largest database of trusted experimental protocols

Dulbecco modified eagle medium (dmem)

Manufactured by Corning
Visit Supplier
Sourced in United States, China, Germany, Canada, New Zealand, Switzerland, Colombia, United Kingdom, France, Holy See (Vatican City State), Italy, Morocco, Poland

DMEM (Dulbecco's Modified Eagle Medium) is a cell culture medium used to support the growth and maintenance of various cell types in vitro. It provides the necessary nutrients, minerals, and other components required for cell proliferation and survival. DMEM is a widely used basal medium in the field of cell biology and biotechnology.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (543 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (294 mentions) Penicillin streptomycin, by Corning (265 mentions) Fetal bovine serum (fbs), by Corning (262 mentions) Rpmi 1640, by Corning (177 mentions) Penicillin, by Thermo Fisher Scientific (153 mentions) Fetal bovine serum (fbs), by Merck Group (140 mentions) Streptomycin, by Thermo Fisher Scientific (139 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (119 mentions) L glutamine, by Corning (100 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (99 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (97 mentions) Glutamax, by Thermo Fisher Scientific (91 mentions) Streptomycin, by Corning (89 mentions) L glutamine, by Thermo Fisher Scientific (89 mentions) Penicillin, by Corning (87 mentions) Rpmi 1640 medium, by Corning (86 mentions) Hek293t cell, by American Type Culture Collection (69 mentions) Roswell park memorial institute (rpmi), by Corning (60 mentions) Mda mb 231, by American Type Culture Collection (59 mentions)

2 600 protocols using dulbecco modified eagle medium (dmem)

1

Culturing MEFs, T98G, HEK293T, and NIH 3T3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MEFs (both males and females) were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), 2.5 μg/mL Puromycin (GIBCO), and 50 μg/mL Hygromycin B (Invitrogen). T98G cells (glioblastoma cells from a male individual, obtained from ATCC) were cultured in DMEM (Cellgro), 10% fetal bovine serum, and 1% Penicillin/Streptomycin (Cellgro). T98G cells with RNAi were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), and 1 μg/mL Puromycin (GIBCO). T98G cells engineered with the FUCCI probes and pTRIPZ-EMI1-FLAG were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), 1 μg/mL Puromycin (GIBCO), 150 μg/mL Hygromycin B (Invitrogen), and 10 μg/mL Blasticidin (Invivogen). HEK293T cells (from a female fetus) cultured in DMEM (Cellgro), 10% donor calf serum, and 1% Penicillin/Streptomycin (Cellgro). NIH 3T3 cells were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), and 2.5 μg/mL Puromycin (GIBCO). 0.5 μg/mL doxycycline was the working concentration for all doxycycline systems. All cultures were maintained in 5% CO2 at 37°C. Regarding mice use for the generation of MEFs, all animal work was approved by the NYULMC Institutional Animal Care & Use committee.
Bainor A.J., Saini S., Calderon A., Casado-Polanco R., Giner-Ramirez B., Moncada C., Cantor D.J., Ernlund A., Litovchick L, & David G. (2018). The HDAC-Associated Sin3B Protein Represses DREAM Complex Targets and Cooperates with APC/C to Promote Quiescence. Cell reports, 25(10), 2797-2807.e8.
+ Open protocol
+ Expand
2

Culturing MEFs, T98G, HEK293T, and NIH 3T3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MEFs (both males and females) were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), 2.5 μg/mL Puromycin (GIBCO), and 50 μg/mL Hygromycin B (Invitrogen). T98G cells (glioblastoma cells from a male individual, obtained from ATCC) were cultured in DMEM (Cellgro), 10% fetal bovine serum, and 1% Penicillin/Streptomycin (Cellgro). T98G cells with RNAi were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), and 1 μg/mL Puromycin (GIBCO). T98G cells engineered with the FUCCI probes and pTRIPZ-EMI1-FLAG were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), 1 μg/mL Puromycin (GIBCO), 150 μg/mL Hygromycin B (Invitrogen), and 10 μg/mL Blasticidin (Invivogen). HEK293T cells (from a female fetus) cultured in DMEM (Cellgro), 10% donor calf serum, and 1% Penicillin/Streptomycin (Cellgro). NIH 3T3 cells were cultured in DMEM (Cellgro), 10% fetal bovine serum, 1% Penicillin/Streptomycin (Cellgro), and 2.5 μg/mL Puromycin (GIBCO). 0.5 μg/mL doxycycline was the working concentration for all doxycycline systems. All cultures were maintained in 5% CO2 at 37°C. Regarding mice use for the generation of MEFs, all animal work was approved by the NYULMC Institutional Animal Care & Use committee.
Bainor A.J., Saini S., Calderon A., Casado-Polanco R., Giner-Ramirez B., Moncada C., Cantor D.J., Ernlund A., Litovchick L, & David G. (2018). The HDAC-Associated Sin3B Protein Represses DREAM Complex Targets and Cooperates with APC/C to Promote Quiescence. Cell reports, 25(10), 2797-2807.e8.
+ Open protocol
+ Expand
3

Culturing Diverse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 2

In a cell culture method in accordance with Example 2, four kinds of cell lines were divided to respective media and then cultured in a confluent manner in a 5% CO2 incubator at 37° C. In this case, lung cancer cell A549 (purchased from ATCC) contained 10% FetalClone III (Lonza) and 1% penicillin-streptomycin (MP) in DMEM (Cellgro); lung cancer cell A549D9K (D9K mutated CXCR2 expressed in A549) contained 10% FetalClone III (Lonza), 1% penicillin-streptomycin (MP), and 600 μg/mL of G418 (Cellgro) in DMEM (Cellgro); osteoblast MC3T3 (purchased from ATCC) contained 10% fetal bovine serum (FBS, Lonza) and 1% penicillin-streptomycin (MP) in MEM Alpha Modification (HyClone); and fibroblast NIH3T3 (purchased from ATCC) and kidney cell HEK293 (purchased from ATCC) contained 10% fetal bovine serum (FBS, Lonza) and 1% penicillin-streptomycin (MP) in DMEM (Cellgro).

US09908089B2. Device for producing microbubble water by using ultrasonic vibrator, cell culture medium containing microbubble water, cell culturing method using same, high efficiency mixed fuel using microbubbles, and method for manufacturing same (2018-03-06). CHUNG-ANG UNIVERSITY INDUSTRY-ACADEMY COOPERATION FOUNDATION [KR]. Inventors: Jong Min Kim [KR], Seung Hoon Oh [KR].
+ Open protocol
+ Expand
4

Murine Bone Marrow Cell Isolation and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine bone marrow macrophages (BMM) were differentiated from the bone marrow of six to eight week old female or male C57BL/6J mice and maintained in culture with bone marrow macrophage medium (BMM medium - DMEM (CellGro) + 1 mM Sodium Pyruvate (CellGro) + 1 mM HEPES Buffer (CellGro) + 2 mM L-glutamine (CellGro) + 20% heat inactivated FBS (Seradigm) + 30% L929 fibroblast cell supernatant + 100 μg/ml Penicillin/Streptomycin (Pen/Strep) (CellGro) + 50 μM β-mercaptoethanol (Amresco) at 37°C, 5% CO2. Murine bone marrow neutrophils were isolated from the bone marrow of six to eight week old female or male C57BL/6J mice and maintained in culture with DMEM (Corning) + 10% heat inactivated FBS supplemented with 100 μg/ml Penicillin/Streptomycin at 37°C, 5% CO2. NFκB reporter macrophages (RAW-Blue – Invivogen) were maintained in culture with DMEM (Corning) + 10% heat inactivated FBS supplemented with 100 μg/ml Penicillin/Streptomycin at 37°C, 5% CO2.
Grayczyk J.P., Harvey C.J., Laczkovich I, & Alonzo F I.I.I. (2017). A Lipoylated Metabolic Protein Released by Staphylococcus aureus Suppresses Macrophage Activation. Cell host & microbe, 22(5), 678-687.e9.
+ Open protocol
+ Expand
5

Culturing and Differentiating Kidney Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
LLC-PK1 cells were purchased from the ATCC (CL-101) and cultured in DMEM (Corning Cellgro, NY) supplemented with 10% FBS (HyClone, MA) and 1% penicillin/streptomycin (Corning Cellgro, NY) at 37 °C in a humidified, 5% CO2 atmosphere. The cell line was confirmed to be mycoplasma-free with repeated testing, using a mycoplasma detection kit (MycoAlert, Lonza, Switzerland). Prior to the experiments, antibiotics were withdrawn, and cells were serum-starved for 24 h to induce differentiation. In some experiments, primary culture endothelial cells were generated from Tie2Cre•Pkd2WT/WT mouse aortas, as previously described.4 (link) Prior to the experiments, these cells were cultured in DMEM (Corning Cellgro, NY) containing 10% FBS (HyClone, MA) at 39 °C in a humidified, 5% CO2 atmosphere.
Pala R., Mohieldin A.M., Sherpa R.T., Kathem S.H., Shamloo K., Luan Z., Zhou J., Zheng J.G., Ahsan A, & Nauli S.M. (2019). Ciliotherapy: Remote Control of Primary Cilia Movement and Function by Magnetic Nanoparticles. ACS nano, 13(3), 3555-3572.
+ Open protocol
+ Expand
6

Cell Culture Conditions for Multiple Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human cell lines EJ-1 (CRL-3380, ATCC), HCT 116 (CCL-247, ATCC), MCF7 (HTB-22, ATCC), MDA-MB-231 (HTB-26, ATCC), NCI-H157 (CRL-5802, ATCC), NCI-H292 (CRL-1848, ATCC), NCI-H460 (HTB-177, ATCC), NCI-H661 (HTB-183, ATCC), and T24 (HTB-4, ATCC) were cultured in RPMI-1640 medium (Cellgro, Corning). Human 801-D (200690YJ, KCB, Kunming, China), HEK-293T (CRL-11268, ATCC), HeLa (CCL-2, ATCC), HLF-a (CCL-199, ATCC), HT-29 (HTB-38, ATCC), HUVEC (PCS-100-013, ATCC), LX-2 (337957, BNCC, Beijing, China), SK-MES-1 (HTB-58, ATCC), T98G (CRL-1690, ATCC), and U-87 MG (HTB-14, ATCC) cells were maintained in Dulbecco's modified Eagle medium (DMEM, Cellgro). Human A549 (CCL-185, ATCC), HepG2 (HB-8065, ATCC), K-562 (CCL-243, ATCC), MG-63 (CRL-1427, ATCC), PANC-1 (CRL-1469, ATCC), SaOS2 (HTB-85, ATCC), U2OS (HTB-96, ATCC), and NSC cells were maintained in DMEM: Nutrient Mixture F-12 (Cellgro). The cell lines GBM-18 and GBM-3 were cultured in DMEM (Cellgro).
All culture media was supplemented with 10% fetal bovine serum, 100 unit/mL penicillin and 100 unit/mL streptomycin, and were maintained in a humidified atmosphere containing 5% CO2 in an incubator at 37 °C.
Wang G., Zhang M., Meng P., Long C., Luo X., Yang X., Wang Y., Zhang Z., Mwangi J., Kamau P.M., Dai Z., Ke Z., Zhang Y., Chen W., Zhao X., Ge F., Lv Q., Rong M., Li D., Jin Y., Sheng X, & Lai R. (2022). Anticarin-β shows a promising anti-osteosarcoma effect by specifically inhibiting CCT4 to impair proteostasis. Acta Pharmaceutica Sinica. B, 12(5), 2268-2279.
+ Open protocol
+ Expand
7

Cell Culture Conditions for Various Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
All media described below were supplemented with 10% fetal bovine serum and 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco). Human middle ear epithelial cells (HMEECs) were maintained in DMEM (Cellgro) supplemented with BEGM SingleQuots (Lonza). Lung epithelial A549 cells were maintained in F-12K medium (Gibco). Human cervical epithelial HeLa cells were maintained in DMEM (Cellgro). Cells were cultured at 37 °C in a humidified 5% CO2 atmosphere.
Konduru A.S., Lee B.C, & Li J.D. (2016). Curcumin suppresses NTHi-induced CXCL5 expression via inhibition of positive IKKβ pathway and up-regulation of negative MKP-1 pathway. Scientific Reports, 6, 31695.
+ Open protocol
+ Expand
8

Maintenance of MCL and MEF Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three MCL cell lines (Granta-519, JeKo-1 and Mino) were used in this study. Granta-519 was maintained in Dulbecco modified Eagle medium with high glucose (DMEM, Cellgro) supplemented with 20% FBS while both Jeko-1 and Mino cell lines were cultured in RPMI 1640 medium (ATCC) supplemented with 20% FBS. Wild type, heterozygote and double knockout mouse embryonic fibroblasts for Casp3 and Casp7 (DKO MEF) were maintained through passage 10 in DMEM (Cellgro) supplemented with 10% FBS. All media were supplemented with 1% penicillin-streptomycin (Invitrogen Inc.). These cell lines were routinely tested for Mycoplasma using a MycoTect kit (Invitrogen). MCL cell lines were validated by AmpF/STR Identification kit (Applied Biosystems) in the MD Anderson cell line validation core facility. Routine cell number and the mean cell volume were determined by Coulter channelyzer (Coulter Electronics).
Sarkar A., Balakrishnan K., Chen J., Patel V., Neelapu S.S., McMurray J.S, & Gandhi V. (2015). Molecular evidence of Zn chelation of the procaspase activating compound B-PAC-1 in B cell lymphoma. Oncotarget, 7(3), 3461-3476.
+ Open protocol
+ Expand
9

Cell Culture Protocols for Breast Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDA-MB-231, Hs578t, MCF-7, and MDA-MB-468 cells were purchased from American Type Culture Collection (ATCC). MDA-MB-231 and MCF-7 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Corning CellGro., Manassas, VA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), 1% pen–strep, and 1% L-glutamine (Corning, CellGro). MDA-MB-468 was cultured in DMEM (Corning CellGro) supplemented with 10% fetal bovine serum, 1% pen–strep, 1% L-glutamine, 1% sodium pyruvate, and 1% Minimum Essential Medium (MEM) amino acids (Corning CellGro). Hs578t was cultured in MEM supplemented with 10% fetal bovine serum, 1% pen–strep, and 1% L-glutamine (Corning CellGro). SUM159PT cells were obtained from Asterand and cultured in Ham’s/F-12 (Corning CellGro) supplemented with 10% fetal bovine serum, 1% pen–strep, 1% L-glutamine, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1 μg/mL hydrocortisone, and 5 μg/mL insulin. All cell lines were maintained in a humidified incubator at 5% CO2 and 37 °C.
Andrade D., Mehta M., Griffith J., Oh S., Corbin J., Babu A., De S., Chen A., Zhao Y.D., Husain S., Roy S., Xu L., Aube J., Janknecht R., Gorospe M., Herman T., Ramesh R, & Munshi A. (2019). HuR Reduces Radiation-Induced DNA Damage by Enhancing Expression of ARID1A. Cancers, 11(12), 2014.
+ Open protocol
+ Expand
10

Culturing Mammalian Cell Lines

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hek293 cells were maintained in DMEM (Cat#10-013-CV, Cellgro, San Diego, CA) and HeLa, U2OS and Mefs in DMEM (Cat#15-017-CV) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, and penicillin/streptomycin in an atmosphere of 5% CO2 and temperature of 37°C. Mef-wt, Mef-Mfn1 null and Mef-Mfn2 null cells are from Dr. David Chan’s Research Group (Division of Biology and the Howard Hughes Medical Institute, California Institute of Technology, USA) (14 (link)).
Kim C., Juncker M., Reed R., Haas A., Guidry J., Matunis M., Yang W.C., Schwartzenburg J, & Desai S. (2021). SUMOylation of mitofusins: a potential mechanism for perinuclear mitochondrial congression in cells treated with mitochondrial stressors. Biochimica et biophysica acta. Molecular basis of disease, 1867(6), 166104.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!