Unless stated otherwise, all the chemicals and reagents were commercially obtained and used without further purification. Tris(hydroxymethyl)aminomethane (Tris), o-nitrophenyl-β-d -galactopyranoside (ONPG),36 (link) acrylamide, 2,2,2-trifluoroethanol (TFE), 2,2′-azobis[N-(2-carboxyethyl)-2-methylpropionamidine] tetrahydrate (VA-057), disodium phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), and fluorescein were purchased from Wako Pure Chemical Industries, Ltd (Japan). Diacetone acrylamide (DAAM) and adipic dihydrazide (ADH) were purchased from Tokyo Chemical Industry Co., Ltd (Japan). DAAM was re-crystallised several times from ethyl acetate to remove the inhibitor (mequinol) before use in the reversible addition-fragmentation chain transfer (RAFT) polymerisation.28 (link) Lactase,25 (link) nylon6 (Cat. No., 181110), and acetyl cellulose (AcCel; Mn = 30 000) were purchased from Sigma-Aldrich (USA). Poly(AM/DAAM) featuring AM and DAAM combined in a molar ratio of 4 : 1, was synthesised as described in our previous paper17 (link) by replacing the poly(AM/DAAM) with PAM-co-PDAAM. The FITC-labelled lysozyme was synthesised using the method described in a previous study.1 (link) The Mn and polydispersity index (PDI) of the poly(AM/DAAM) were calculated from the GPC profile calibrated using poly(ethylene glycol) (PEG) standards.
Sodium dihydrogen phosphate nah2po4
Manufactured by Fujifilm
Sourced in Japan
Sodium dihydrogen phosphate (NaH2PO4) is a chemical compound used in various laboratory applications. It is a colorless, crystalline solid that is soluble in water. The primary function of sodium dihydrogen phosphate is to serve as a buffer agent, maintaining a specific pH level in aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Disodium hydrogen phosphate na2hpo4, by Fujifilm (3 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (2 mentions)
Phosphate buffered saline tablet, by Merck Group (2 mentions)
Acrylamide, by Fujifilm (1 mentions)
Fluorescein, by Fujifilm (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Fujifilm (1 mentions)
Recombinant human insulin, by Fujifilm (1 mentions)
Dna quantification kit, by Merck Group (1 mentions)
Cellstain double staining kit, by Dojindo Laboratories (1 mentions)
L cysteine hydrochloride monohydrate, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Papain, by Merck Group (1 mentions)
Micro bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Doxorubicin hydrochloride, by Tokyo Chemical Industry (1 mentions)
Genipin, by Fujifilm (1 mentions)
Sucrose, by Fujifilm (1 mentions)
Paraformaldehyde solution, by Fujifilm (1 mentions)
Mayer s hematoxylin solution, by Fujifilm (1 mentions)
5 protocols using sodium dihydrogen phosphate nah2po4
1
Reversible Polymer Synthesis and Characterization
2
Collagen-based Biomaterial Fabrication
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Pepsin-solubilized collagen (PSC) at pH 3 was diluted in HCl (1 % solution of mainly type I collagen from porcine skin, Nipponham, Japan). Acid-solubilized collagen at pH 3 was diluted in HCl (0.3 % solution of mainly type I collagen from porcine tendon, Nitta Gelatin, Japan). Genipin (Ge; Wako Pure Chemical Industries, Japan), phosphate buffered saline (PBS) tablets (Sigma-Aldrich, MO), disodium hydrogen phosphate (Na
2HPO
4; Wako Pure Chemical Industries, Japan), sodium dihydrogen phosphate (NaH
2PO
4; Wako Pure Chemical Industries, Japan), NIH/3T3 fibroblast cells (RIKEN RCB2767), Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, MO), calf serum (CS; MP Biomedicals, Germany), antibiotic-antimycotic (Gibco, NY), Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Japan), 0.4 % SH (MucoUp, Boston Scientific Japan, Japan), 5 % DW and NS were purchased and used without further purification.
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Pepsin-solubilized collagen (PSC) at pH 3 was diluted in HCl (1 % solution of mainly type I collagen from porcine skin, Nipponham, Japan). Acid-solubilized collagen at pH 3 was diluted in HCl (0.3 % solution of mainly type I collagen from porcine tendon, Nitta Gelatin, Japan). Genipin (Ge; Wako Pure Chemical Industries, Japan), phosphate buffered saline (PBS) tablets (Sigma-Aldrich, MO), disodium hydrogen phosphate (Na
2HPO
4; Wako Pure Chemical Industries, Japan), sodium dihydrogen phosphate (NaH
2PO
4; Wako Pure Chemical Industries, Japan), NIH/3T3 fibroblast cells (RIKEN RCB2767), Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, MO), calf serum (CS; MP Biomedicals, Germany), antibiotic-antimycotic (Gibco, NY), Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Japan), 0.4 % SH (MucoUp, Boston Scientific Japan, Japan), 5 % DW and NS were purchased and used without further purification.
3
PLGA-Based Insulin Delivery System
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PLGA (copolymer composition ratio of 50 : 50, weight average molecular weight of 20 kDa, and inherent viscosity of 0.187 to 0.229 dL/g), methylene chloride (CH2Cl2), polyvinyl alcohol (86–90 mol% hydrolysis), recombinant human insulin, hydrochloric acid (HCl), sodium hydroxide (NaOH), absolute ethanol (99.5%), N-hydroxysuccinimide esters (NHS), 25% glutaraldehyde solution, and sodium dihydrogen phosphate (NaH2PO4) were obtained from Wako Pure Chemicals Ltd., Japan. L-cysteine hydrochloride monohydrate (minimum 98%), ethylene diamine tetra acetic acid (EDTA), papain, DNA quantification kit, Dulbecco's Modified Eagle's Medium (DMEM), growth supplements, and antibiotics were obtained from Sigma-Aldrich, USA. Phosphate buffer saline (10x, pH = 7.4) was obtained from Nacali Tesque Inc., Japan. Porcine collagen type-1 was obtained from Nitta Gelatin, Japan. 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC/EDAC) was obtained from Peptide Institute Inc., Japan. Cellstain Double Staining Kit was obtained from Dojindo Laboratories, Japan. Micro BCA protein assay Kit was obtained from Pierce Biotechnology, USA. All the materials in this study were used as received without further purification. Molecular biology grade milli-Q water from millipore water system (Millipore Corporation, USA) was used for preparation of all the solutions and reagents.
4
Polysaccharide-based Drug Delivery System
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In this study, five kinds of polysaccharides
such as cellulose, chitin, chitosan, and cellulose nanocrystal were
used. Each polysaccharide, cellulose, chitin, and chitosan we purchased
was composed of nanofibers. A cellulose solution (2 wt % in water),
a chitin solution (2 wt % in water), and a chitosan solution (2 wt
% in water) were obtained from Sugino Machine Limited (Japan). A cellulose
nanocrystal (2 wt % in water) was purchased from DKS Co. Ltd. (Japan).
Dimethyl sulfoxide (DMSO, >99.0 wt %) and PBS (0.1 M, pH = 7),
prepared
using sodium dihydrogen phosphate (NaH2PO4)
and disodium hydrogen phosphate (Na2HPO4) were
purchased from FUJIFILM Wako Pure Chemical Corporation (Japan). Doxorubicin
hydrochloride (>95.0%) as a model cancer cell treatment drug was
purchased
from Tokyo Chemical Industry Co., Ltd. (Japan). MMT was purchased
from Sigma-Aldrich (USA). All the chemicals were used without further
purification. The skin of a 7 week-old male hairless mouse, from which
subcutaneous fat was removed, was purchased from Japan SLC, Inc. (Japan).
such as cellulose, chitin, chitosan, and cellulose nanocrystal were
used. Each polysaccharide, cellulose, chitin, and chitosan we purchased
was composed of nanofibers. A cellulose solution (2 wt % in water),
a chitin solution (2 wt % in water), and a chitosan solution (2 wt
% in water) were obtained from Sugino Machine Limited (Japan). A cellulose
nanocrystal (2 wt % in water) was purchased from DKS Co. Ltd. (Japan).
Dimethyl sulfoxide (DMSO, >99.0 wt %) and PBS (0.1 M, pH = 7),
prepared
using sodium dihydrogen phosphate (NaH2PO4)
and disodium hydrogen phosphate (Na2HPO4) were
purchased from FUJIFILM Wako Pure Chemical Corporation (Japan). Doxorubicin
hydrochloride (>95.0%) as a model cancer cell treatment drug was
purchased
from Tokyo Chemical Industry Co., Ltd. (Japan). MMT was purchased
from Sigma-Aldrich (USA). All the chemicals were used without further
purification. The skin of a 7 week-old male hairless mouse, from which
subcutaneous fat was removed, was purchased from Japan SLC, Inc. (Japan).
5
Porcine Collagen Scaffold Fabrication
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Pepsin-digested collagen from porcine skin in dilute HCl (pH 3; 1% solution of mainly type I collagen; Nipponham Foods Ltd, Osaka, Japan), genipin (Wako Pure Chemical Industries, Ltd., Osaka, Japan), phosphate-buffered saline tablets (Sigma-Aldrich Co., St Louis, MO, USA), disodium hydrogen phosphate (Na2HPO4; Wako Pure Chemical Industries, Ltd.), sodium dihydrogen phosphate (NaH2PO4; Wako Pure Chemical Industries, Ltd.), sodium chloride (NaCl; Wako Pure Chemical Industries, Ltd.), 4% paraformaldehyde solution (Wako Pure Chemical Industries, Ltd.), sucrose (Wako Pure Chemical Industries, Ltd.), 4% carboxymethylcellulose (CMC) sodium salt (Leica Microsystems, Wetzlar, Germany), ethanol (Wako Pure Chemical Industries, Ltd.), Mayer’s hematoxylin solution, 0.5% eosin Y ethanol solution (Wako Pure Chemical Industries, Ltd.), and raw porcine stomach (Tokyo Shibaura Zouki Co., Tokyo, Japan) were purchased.
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