Inbred C57BL/6JJ female mice were purchased from Japan SLC, Inc. (Hamamatsu, Japan). Because a previous study indicated that female mice were more sensitive than male mice to vitamin D supplementation by UV irradiation24 (link), we used only female mice in the present study. They were kept at a temperature of 25 °C with a 12-h light–dark cycle and shielded from UVB of an ordinary fluorescent light. To create a 25-hydroxyvitamin D [25(OH)D]-starved mouse group for the experiments, mice were weaned from their mothers and fed the standard wheat-based mouse diet until 12 weeks of age. Then they were fed a vitamin D-deficient diet (AIN93GA-2, Oriental Yeast Co ltd., Tokyo, Japan) until the study termination25 (link), at 32 weeks of age. AIN93GA-2 contains no vitamin D, 0.50% calcium, 0.20% phosphorus, and 7.00% total fat.
Ain93ga 2
Manufactured by Oriental Yeast
Sourced in Japan
The AIN93GA-2 is a laboratory equipment product designed for research and testing purposes. It serves as a specialized tool for conducting experiments and analyses. The core function of this equipment is to provide a controlled environment for various scientific applications, but its specific intended use is not included in this factual description.
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Lab products found in correlation
4 protocols using ain93ga 2
1
Vitamin D-Deficient Mouse Model
2
Postmenopausal Osteoporosis Mouse Model
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Inbred C57BL/6 female mice were obtained from Japan SLC, Inc. (Hamamatsu, Japan). The mice were shielded from normal fluorescent UVB in a 12 h light–dark cycle, and kept at a temperature of 25° C. Mice were fed a standard wheat-based diet until the age of 12 weeks. To make an experimental 25 (OH) D starved mouse group, Mice were fed AIN93G as a vitamin D-containing diet and AIN93GA-2 (Oriental Yeast Co., Ltd., Tokyo, Japan) as a vitamin D-deficient diet until 24 weeks of age when the study protocol was completed28 (link). AIN93G contains 1000 IU / kg of vitamin D, 0.50% calcium and 7.00% total fat, whereas AIN93GA-2 contains 0 IU / kg, 0.50% and 7.00%, respectively. To create the postmenopausal osteoporosis model, we ovariectomized mice at 16 weeks of age as described in the previous reports27 (link),29 . Finally, the mice were randomly divided into four groups (n = 6): (1) Vit.D+ OVX− as normal control; (2) Vit.D+ OVX+ ; (3) Vit.D− OVX− ; (4) Vit.D− OVX+ .
3
Vitamin D-Deficient Diet Effects
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SAMP6 were purchased from Japan SLC, Inc. (Hamamatsu, Japan) after approval was obtained from the Council for SAM Research (Kyoto, Japan). We used only female mice also for the preliminary experiments. Then they were fed a vitamin D-deficient diet or a vitamin D-replete diet (AIN93GA-2, Oriental Yeast Co ltd., Tokyo, Japan) from 12 weeks of age until the study termination.
4
Vitamin D3 and MCT Oil Supplementation in Mice
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Reagents and administration methods. We used vitamin D3 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and medium chain trilcerides (MCT) oil (Nisshin OilliO Group, Tokyo, Japan). After checking UV absorption spectrum of vitamin D3, we dissolved these reagents in MCT oil at the indicated concentration. They were administered to mice using a feeding tube (FTSS-20S-25, Prime Bioscience, Singapore) every day for 4 wk. Each volume for oral administration was 200 mL.
Animals. Ten-week-old C57BL/6J male mice obtained from Charles River Laboratories (Tokyo, Japan) were maintained in an environmentally controlled clean room at Saitama Medical University. The experiments were conducted according to the institutional guidelines for ethical animal experiments (No. 2482, 2751, 2989). These mice were fed a normal diet (AIN93G, Oriental Yeast Co., Ltd., Tokyo, Japan) or vitamin D deficient diet (AIN93GA-2, Oriental Yeast Co., Ltd.) from 0 wk of age for 8 wk. We also measured body weight at the following points; just before the first administration (0 w), 1 wk (1 w), 2 wk (2 w), and 4 wk after the first administration (4 w). Mice were randomly divided into groups with MCT or graded doses of vitamin D3. We showed experimental groups and protocol in Table S1 and Fig. S1 (Supplemental Online Material).
Animals. Ten-week-old C57BL/6J male mice obtained from Charles River Laboratories (Tokyo, Japan) were maintained in an environmentally controlled clean room at Saitama Medical University. The experiments were conducted according to the institutional guidelines for ethical animal experiments (No. 2482, 2751, 2989). These mice were fed a normal diet (AIN93G, Oriental Yeast Co., Ltd., Tokyo, Japan) or vitamin D deficient diet (AIN93GA-2, Oriental Yeast Co., Ltd.) from 0 wk of age for 8 wk. We also measured body weight at the following points; just before the first administration (0 w), 1 wk (1 w), 2 wk (2 w), and 4 wk after the first administration (4 w). Mice were randomly divided into groups with MCT or graded doses of vitamin D3. We showed experimental groups and protocol in Table S1 and Fig. S1 (Supplemental Online Material).
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