Diethyl pyrocarbonate
Diethyl pyrocarbonate is a chemical compound used in laboratory settings. It functions as a reagent to inactivate RNase enzymes, which are known to degrade RNA samples. This property makes diethyl pyrocarbonate a useful tool in RNA-related experiments and research.
Lab products found in correlation
54 protocols using diethyl pyrocarbonate
RNase-free Experimental Procedures
Optimized Cryopreservation of Mouse Brain Sections for LCM
Reagents and Oligonucleotides for Telomere Assay
hexacyanoferrate(III) (99.98%),
potassium hexacyanoferrate(II) trihydrate (≥99.95%), ethylenediaminetetraacetic
acid (EDTA) ACS reagent (99.4–100.6%), 2-propanol anhydrous
(99.5%), sodium chloride (≥99.95%), acetic acid (≥99%),
Tween 20, Trizma base (99.9%) Trizma hydrochloride (≥99%),
methanol (≥99%), phenylmethanesulfonyl fluoride (PMSF), diethyl
pyrocarbonate (DCEP) (≥97%),
(≥98%), glycine (≥99%), sodium dodecyl sulfate (SDS;
≥98.5%), and ddGTPs were purchased from Sigma-Aldrich (St.
Louis, MO). Acrylamide (40%), ammonium persulfate, tetramethylethylenediamine,
4× Laemmli sample buffer, and precision plus protein standard
were obtained from BioRad (Hercules, CA). Tris(2-carboxyethyl) phosphine
hydrochloride (TCEP), sulfuric acid optima (93–98%), Coomassie
Brilliant Blue G-250, HEPES buffer, detergent-compatible Bradford
assay kit, and RPMI-164 medium + 2.05 mM glutamine (Hyclone) were
purchased from Fisher Scientific (Fair-lawn, NJ). All synthetic oligonucleotides
were purchased from Integrated DNA Technology (IDT) (San Diego, CA)
with the following sequences:
and
dNTPs were purchased
from Promega (Madison, WI, USA).
Antimalarial Compounds Evaluation
Porous Hydrogel Scaffold Fabrication
skin (bloom 300), GA (25% solution in water), glycine, 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimidehydrochloride
(EDC), diethylpyrocarbonate, glycyrrhetinic acid, and collagenase
from Clostridium histolyticum in addition
to the chemicals that were used in the cytotoxicity studies: dimethyl
sulfoxide (DMSO), MTT, and trypan blue dye were purchased from Sigma,
St. Louis, Mo., USA. Acetone was purchased from Labscan, Dublin, Ireland.
Allicin was purchased from Jiangsu chiataiqingjiang Pharmaceutical
Co., Ltd., China. Spectra/Por dialysis membrane, 12 000–14 000
molecular weight cutoff, was purchased from Spectrum Laboratories
Inc., Rancho Dominguez, Canada.
Molecular Mechanism of Apoptosis Induction
Quantification of Katanin p60 Expression
Preparation and Immunostaining of Tissue Scaffolds
Low-temperature enzymatic antigen demasking was performed using pepsin, hyaluronidase, and proteinase K. Slides were either incubated with pepsin (0.1% in diethylpyrocarbonate; Sigma-Aldrich) for 30 min, hyaluronidase (0.02% in phosphate-buffered saline and 0.01% bovine serum albumin; Sigma-Aldrich) for 20 min, or proteinase K (5% in TE-Buffer pH 8; Roche) for 15 min at 37°C. Alternatively, heat-induced antigen retrieval was performed in a KOS microwave (Milestone) with Target Retrieval Solution pH 9 (Dako) at 93°C for 15 min. After blocking in H2O2 Block (ThermoScientific) for 10 min and UV Block (ThermoScientific) for 5 min, slides were exposed to anti-vimentin (0.078 μg/ml; Dako) for 30 min to detect hAMSCs on the carriers. Slides were developed using the UltraVision LP Detection System (ThermoScientific).
Sampling and Extraction of Placental Tissues
Chorionic villus samples from subjects at the first or early second trimesters of pregnancy were collected by chronic villus sampling (CVS). Placenta villi samples (fetal side) were collected from third trimester of pregnancy after delivery. All tissue samples were washed with diethylpyrocarbonate (Sigma-Aldrich, USA) treated water. For DNA analysis, tissues were stored at -80°C. For RNA analysis, tissues were incubated with RNAlater (Life Technologies, USA) at 4°C overnight, and then stored at -80°C. Genomic DNA extraction from tissues was performed with QIAamp DNA Mini Kit (QIAGEN GmbH, Germany), according to manufacturer’s instructions. Total RNA was extracted from frozen tissues using TRIZOL protocol (Life Technologies).
Lentiviral Vector Production in HEK293FT Cells
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