Hematoxylin eosin

Manufactured by Carl Roth

Sourced in Germany

Hematoxylin-Eosin is a commonly used staining technique in histology and pathology. It is a combination of two dyes, hematoxylin and eosin, which stain cell nuclei and cytoplasmic structures, respectively. This staining method provides a clear contrast between different cellular components, enabling the visualization and analysis of tissue samples under a microscope.

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Lab products found in correlation

Normal goat serum (ngs), by Jackson ImmunoResearch (1 mentions) Direct red 80, by Merck Group (1 mentions) Paraformaldehyde pbs, by Merck Group (1 mentions) Alexafluor 488 or 555 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Mayer s haematoxylin, by Carl Roth (1 mentions) Moma 2, by Abcam (1 mentions) Oil red o, by Carl Roth (1 mentions) Masson s trichrome, by Merck Group (1 mentions) Oil red o, by Merck Group (1 mentions) Tissue tek oct compound, by Sakura Finetek (1 mentions) Paraffin, by Thermo Fisher Scientific (1 mentions) Alkaline phosphatase conjugated goat anti rabbit secondary antibody, by Vector Laboratories (1 mentions) Microm hm 340e, by Thermo Fisher Scientific (1 mentions) Aperio genie, by Leica (1 mentions) Aperio imagescope, by Leica (1 mentions) Aperio cs2, by Leica (1 mentions) Hematoxylin, by Carl Roth (1 mentions) Roti mount, by Carl Roth (1 mentions)

Close spelling variants of this product

Hematoxylin and eosin (10 citations) Hematoxylin (34 citations)

7 protocols using hematoxylin eosin

Histological Analysis of Horse Skin

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Horse forehead skin was dissected, washed in PBS, fixed in 4% PFA overnight, dehydrated, embedded in paraffin and sectioned in thickness of 2 µm. After deparaffinization and rehydration, a standard hematoxylin-Eosin staining (hematoxylin-Eosin, Carl Roth GmbH, Karlsruhe, Germany) was carried out. Additionally, Fontana Masson staining was performed as a special stain showing the argentaffin melanocytes. For this, the sections were incubated in ammoniac silver solution (Carl Roth GmbH, Karlsruhe, Germany) at 60 • C for 60 min. After rinsing, the sections were incubated in Gold Chloride Solution (0.2%, Carl Roth GmbH, Karlsruhe, Germany) for 10 min, and sequentially in Sodium Thiosulfate Solution (5%, Carl Roth GmbH, Karlsruhe, Germany) for 2 min. The nuclei were counterstained using hematoxylin (Carl Roth GmbH, Karlsruhe, Germany), and mounted with ROTI ® Mount (Carl Roth GmbH, Karlsruhe, Germany).
In order to assess the number of horse hair follicles per cm 2 of skin, microscopic sections (layer thickness 5 µm) were produced from skin samples. Using five randomly selected areas per specimen, the number of follicles on a surface of 0.5 cm 2 was determined manually.
Stained sections were imaged using Keyence BZ-9000 microscope (Keyence GmbH, Neu-Isenburg, Germany) or Nikon TE2000S (Nikon GmbH, Düsseldorf, Germany).

Li H., Michler J.K., Bartella A.K., Sander A.K., Gaus S., Hahnel S., Zimmerer R., Simon J., Savković V., & Lethaus B. (2021). Culturing of Melanocytes from the Equine Hair Follicle Outer Root Sheath. Processes, 9(1), 177-177.

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Histological and Immunofluorescence Analysis of Cardiac Tissue

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Histology and immunofluorescence staining were performed as described^{58 (link)}. Hearts were excised, rinsed in cold PBS and fixed in 4% paraformaldehyde/PBS (Sigma Aldrich) for 24–48 hours. The tissue was subsequently dehydrated through an increasing ethanol series, cleared in toluol and embedded in paraffin. 5 µm paraffin sections were stained with hematoxylin & eosin (Carl Roth) to assess overall cardiac morphology or with Sirius Red (Direct Red 80, Sigma Aldrich) to visualize myocardial fibrosis.
For immunofluorescence staining paraffin sections were deparaffinized, rehydrated and heat mediated antigen retrieval was performed in sodium citrate buffer (10 mM, pH 6.0) for 20 minutes. After blocking in antibody solution containing 5% normal goat serum (Jackson ImmunoResearch) for 1 hour, sections were incubated over night with primary antibodies at 4 °C. Secondary antibody detection was performed at room temperature for 1 hour using Alexa Fluor 488 or 555 conjugated secondary antibodies (Life Technologies, 1:500). Nuclei were stained with DAPI or TO-PRO-3 (Life Technologies) and sections were mounted in ProLong Gold antifade reagent (Life Technologies).

Hennig M., Ewering L., Pyschny S., Shimoyama S., Olecka M., Ewald D., Magarin M., Uebing A., Thierfelder L., Jux C, & Drenckhahn J.D. (2019). Dietary protein restriction throughout intrauterine and postnatal life results in potentially beneficial myocardial tissue remodeling in the adult mouse heart. Scientific Reports, 9, 15126.

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Histological Analysis of Aortic Atherosclerosis

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The aortic root was embedded in Tissue-Tek O.C.T. Compound (Sakura Finetek, Alphen aan den Rijn, Netherlands) and snap-frozen in a methylbutane bath cooled with dry ice. Cryosections of 5 µm were obtained and used for hematoxylin–eosin (Carl Roth, Karlsruhe, Germany), Masson's trichrome (Sigma-Aldrich, St. Louis, MO, USA) as well as monocyte and macrophage (MOMA-2; ab33451, Abcam, Cambridge, UK) and myeloid cell (CD11b; 101,202, BioLegend, San Diego, CA, USA) stainings according to the manufacturer’s recommendations. For Oil Red O staining a 0.5% (w/v) stock solution of Oil Red O (Sigma-Aldrich, St. Louis, MO, USA) in isporopanol (> 99.8%) was filtered through Whatman paper. A working solution was prepared by mixing 6 parts of stock solution with 4 parts of water. Lipids were stained in freshly filtered working solution of Oil Red O, cell nuclei in Mayer's Haematoxylin (Carl Roth, Karlsruhe, Germany). Atherosclerotic plaque formation was assessed investigating all cryosections including the cusps of the aortic valve (≥ 5 sections per animal) by an investigator blinded for the genotype. The mean plaque burden of every mouse was calculated and used for further analyses. CD11b and MOMA-2 stainings were analyzed by quantifying the positive area per total plaque area.

Winkler M.J., Müller P., Sharifi A.M., Wobst J., Winter H., Mokry M., Ma L., van der Laan S.W., Pang S., Miritsch B., Hinterdobler J., Werner J., Stiller B., Güldener U., Webb T.R., Asselbergs F.W., Björkegren J.L., Maegdefessel L., Schunkert H., Sager H.B, & Kessler T. (2020). Functional investigation of the coronary artery disease gene SVEP1. Basic Research in Cardiology, 115(6), 67.

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Immunohistochemical Analysis of Pneumococcus

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Whole lungs with trachea were removed, immersion fixed in 4% paraformaldehyde pH 7.0 (Carl Roth, Karlsruhe, Germany) for 48 hours and embedded in paraffin (Thermo Scientific, Waltham, MA). 4 µm thick whole-lung horizontal sections were stained with hematoxylin & eosin (Carl Roth, Karlsruhe, Germany) and scored in a blinded fashion by a veterinary pathologist for the following parameters: perivascular edema, pleuritis, necrosis, area of infection. For immunohistochemical detection of S. pneumoniae in paraffin embedded sections, antigen retrieval was performed with microwave heating (600 W) for 12 minutes in 750 ml 10 mM citric acid pH 6.0 (Merck, Darmstadt, Germany). Sections were incubated with a purified rabbit polyclonal antibody which recognizes epitopes in the capsule, the cell wall and the cytosol of S. pneumoniae (1:2000, kindly provided by Sven Hammerschmidt, Interfaculty Institute for Genetics and Functional Genome Research, University of Greifswald, Germany) at 4°C overnight and subsequently incubated with an alkaline phosphatase conjugated goat anti-rabbit secondary antibody (1:500, Vector, Burlingame, CA) for 30 minutes at room temperature as described in (18 (link)).

Herta T., Bhattacharyya A., Rosolowski M., Conrad C., Gurtner C., Gruber A.D., Ahnert P., Gutbier B., Frey D., Suttorp N., Hippenstiel S, & Zahlten J. (2021). Krueppel-Like Factor 4 Expression in Phagocytes Regulates Early Inflammatory Response and Disease Severity in Pneumococcal Pneumonia. Frontiers in Immunology, 12, 726135.

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Histological Processing of Organ Tissues

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Sections of brain, duodenum, liver and lung were washed in PBS and fixed in 4% formaldehyde (SAV LP, Flinsbach, Germany) for 24 h. Organs were then transferred to PBS and prepared for histology at the Histology Facility of the Joint Technology Platform (Technische Universität Dresden, Biotec, CRTD).
Tissues were embedded into paraffin with the Microm STP 420 D dehydration/infiltration unit (Thermo Fisher Scientific, Waltham, Massachusetts, USA) and the EGF 1160 embedding station (Leica, Wetzlar, Germany). This included stepwise dehydration in a graded alcohol series, transfer to xylol, as well as paraffin infiltration and sample orientation. Paraffin-embedded samples were sectioned using a Microm HM 340E (Thermo Fisher Scientific) and stained with Hematoxylin-Eosin (Carl Roth).

Jühlen R., Peitzsch M., Gärtner S., Landgraf D., Eisenhofer G., Huebner A, & Koehler K. (2018). Compensation for chronic oxidative stress in ALADIN null mice. Biology Open, 7(1), bio030742.

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Cardiac Tissue Histopathological Analysis

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Cardiac cross sections derived from the cardiac base, mid-cavity and apex were fixed with 4% formalin and paraffin-embedded for staining with hematoxylin/eosin (Carl Roth, Germany) and Picrosirius Red (Morphisto, Germany). A blinded expert in veterinary pathology (RK) was asked to grade cellular damage in slides stained with hematoxylin/eosin using a semiquantitative scale ranging from 0 (no damage) to 3 (severe damage). Similarly, cardiomyocyte hypertrophy was graded on a semiquantitative scale as follows: 0 (all cardiomyocytes with regular size in transverse sections), 1 (few cardiomyocytes with increased size in transverse sections), 2 (some cardiomyocytes with increased size in transverse sections), and 3 (majority of cardiomyocytes with increased size in transverse sections). For collagen quantification, histological slides were digitized using an Aperio CS2 image capture device (Leica Biosystems, Germany), and the relative proportion of red-stained collagen from total tissue area was determined by a software algorithm (Aperio ImageScope and Aperio GENIE, both Leica Biosystems). Total collagen in the LV was calculated as the mean of apical, mid-cavity and basal values.

Lohr D., Thiele A., Stahnke M., Braun V., Smeir E., Spranger J., Brachs S., Klopfleisch R., Foryst-Ludwig A., Schreiber L.M., Kintscher U, & Beyhoff N. (2022). Assessment of Myocardial Microstructure in a Murine Model of Obesity-Related Cardiac Dysfunction by Diffusion Tensor Magnetic Resonance Imaging at 7T. Frontiers in Cardiovascular Medicine, 9, 839714.

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Evaluation of Tissue Differentiation

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For evaluation of the differentiation status, Hematoxylin / Eosin staining (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) of cryo-preserved- or paraffin-embedded tissue was performed (see Supplement).

Glowa C., Karger C.P., Brons S., Zhao D., Mason R.P., Huber P.E., Debus J, & Peschke P. (2016). Carbon ion radiotherapy decreases the impact of tumor heterogeneity on radiation response in experimental prostate tumors. Cancer letters, 378(2), 97-103.

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