Tgfb1lox/lox mice, Tgfbr2lox/lox mice, Tgfb1+/− mice, GFAP-Cre mice, and Tlr4−/− mice on C57BL/6 were obtained from Jackson32 (link),61 (link)-64 (link) and continued to be maintained on C57BL/6. CMV-lox-stop-lox-Tgfb1 mice30 (link) obtained from Jackson were backcrossed into C57BL/6. POMC-Cre mice maintained on C57BL/6 were used in our previous research20 (link),65 (link). All mice were housed in standard, pathogen-free animal facility with 12h/12h light and darkness cycles, and adult male mice were used in experiments of this work. Mice were maintained on normal chow since weaning, and for some experiments involving HFD feeding, a HFD (45% kcal fat, Research Diets, Inc.) was used when mice were two to three months old. Food intake and body weight of mice were measured using a laboratory scale. GTT was performed in mice through intraperitoneal (i.p.) injection of glucose at 2g/kg body weight. ITT was performed in mice through i.p. injection of human recombinant insulin (Nova Nordisk) at the dose of 0.7U/kg body weight. Blood glucose levels during GTT and ITT were measured with LifeScan® blood glucose monitoring system. All procedures were approved by the Institutional Animal Care and Use Committee of Albert Einstein College of Medicine.
Blood glucose monitoring system
Manufactured by LifeScan
Sourced in United States
The Blood Glucose Monitoring System is a device used to measure the concentration of glucose in a person's blood. It is a compact, portable device that requires a small blood sample, typically obtained from the fingertip, to provide a reading of the current blood glucose level.
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Lab products found in correlation
5 protocols using blood glucose monitoring system
1
Genetically Modified Mouse Models for Metabolic Studies
2
Genetically Modified Mouse Models for Metabolic Studies
3
Oral Glucose Tolerance Test in Rats
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One day after the end of the intervention period, following 16 h of food deprivation, rats were given an oral gavage of 2 g/kg glucose. Blood was collected at 0, 15, 30, 60, and 120 min post-gavage via tail nick in a chilled tube for insulin analysis. Blood glucose was measured immediately with a blood glucose meter (OneTouch Verio and Blood Glucose Monitoring System, Lifescan, Switzerland). Whole body insulin sensitivity was determined using proxy measures from the glucose tolerance tests (composite insulin sensitivity index – CISI)51 (link).
4
Oral Glucose Tolerance Test in Mice
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Mice were fasted overnight prior to administration of OGTT. The test started at 8 a.m. d -glucose (Sigma, USA) was dissolved in ddH2O and administered orally to the fasted mice (2 g/kg of body weight) using a 20-gauge stainless steel gavage feeding needle. Samples of whole blood (10–20 μl each) were collected from a tail clip bleed immediately before and 15, 30, 60 and, 120 min after administration of glucose. Blood glucose levels were measured using the Blood Glucose Monitoring System (One Touch, Life Scan, USA). The blood samples were taken from vena caudalis, insulin level was measured by insulin ELISA kit (MLbio, China). Total cholesterol (TC) and triglycerides (TG) were determined by enzymatic kits (Applygen, China).
5
Diabetic Retinopathy in Fpr2 Knockout Mice
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Eight‐week‐old C57BL/6 J mice (male, n = 15) and Fpr2−/−mice (male, n = 15) were given five consecutive intraperitoneal injections of streptozotocin (STZ; 60 mg/kg body wt/day) (Sigma‐Aldrich, St. Louis, MO). Control received citrate buffer alone. At 1 week after STZ injection, hyperglycemia was confirmed in tail prick blood samples using Blood glucose monitoring system (LifeScan, CA, USA). Blood glucose values ≥16.7 mmol/L were considered as diabetic and the mice were enrolled into the study. The blood glucose and weight were monitored before and after STZ injection at 1, 4 and 12 and 24 weeks. After injection for 24 weeks, one mouse failed to reach the diabetic standard in diabetic group, two mice failed in Fpr2−/− diabetic group. Twelve mice were survived in diabetic group and 12 mice Fpr2−/− diabetic group. The mice were euthanized to collect retinas for retinal flat‐mount immunofluorescence staining (six retinas), frozen tissue sections immunofluorescence staining (six eyes), RT‐PCR (six retinas), and Western blot (six retinas) experiments.
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