Rabbit anti human p rb

The experimental method has been described previously (Feng et al., 2019b (link)). Briefly, an equal amount of total protein was separated by SDS-PAGE gel and then subjected to other immunoblot analysis by antibody mouse anti-human β-actin (Cat No. bsm-33036M, Bioss, Beijing, China), rabbit anti-human E2F1 (Cat No. ab218527, Abcam, Danvers, MA, United States), rabbit anti-human CDK4 (Cat No. ab108357, Abcam, Danvers, MA, United States), rabbit anti-human Cyclin A2 (Cat No. ab181591, Abcam, Danvers, MA, United States), rabbit anti-human Cyclin D3 (Cat No. ab183338, Abcam, Danvers, MA, United States), rabbit anti-human P-Rb (Cat No. ab184796, Abcam, Danvers, MA, United States), rabbit anti-human CD11b (Cat No. ab133357, Abcam, Danvers, MA, United States), rabbit anti-human CD14 (Cat No. ab133335, Abcam, Danvers, MA, United States), and rabbit anti-human EZH2 (Cat No. ab186006, Abcam, Danvers, MA, United States). Immunoreactive bands were then visualized, and the optical densities were measured.

We first used 8% SDS-PAGE to isolate the protein lysate, and then transferred the protein to 0.22-µm PDVF membrane. PVDF membrane was incubated with the corresponding primary antibody at 4 °C overnight and then incubated with horseradish peroxidase-labeled secondary antibody at room temperature. All the experiments were repeated three times. β-actin (Cat No. bsm-33036M, Bioss, Beijing, China), rabbit anti-human LATS2 (Cat No. 5888, CST, Boston, USA), rabbit anti-human CDK4 (Cat No. ab108357, Abcam, Danvers, MA, USA), rabbit anti-human Cyclin A2 (Cat No. ab181591, Abcam, Danvers, MA, USA), rabbit anti-human Cyclin D3 (Cat No. ab183338, Abcam, Danvers, MA, USA), rabbit anti-human P-Rb (Cat No. ab184796, Abcam, Danvers, MA, USA), rabbit anti-human CD11b (Cat No. ab133357, Abcam, Danvers, MA, USA), rabbit anti-human CD14 (Cat No. ab133335, Abcam, Danvers, MA, USA), and rabbit anti-human EZH2 (Cat No. ab186006, Abcam, Danvers, MA, USA).